Morphological and Molecular Characterization of Heterodera schachtii and the Newly Recorded Cyst Nematode, H. trifolii Associated with Chinese Cabbage in Korea
The sugar beet cyst nematode, is a well known pathogen on Chinese cabbage in the highland fields of Korea. However, a race of cyst forming nematode with close morphological resemblance to was recently isolated from the same Chinese cabbage fields. Morphological species differentiation between the tw...
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Published in | The plant pathology journal Vol. 34; no. 4; pp. 297 - 307 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Korea (South)
한국식물병리학회
01.08.2018
Korean Society of Plant Pathology Hanrimwon Publishing Company |
Subjects | |
Online Access | Get full text |
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Summary: | The sugar beet cyst nematode,
is a well known pathogen on Chinese cabbage in the highland fields of Korea. However, a race of cyst forming nematode with close morphological resemblance to
was recently isolated from the same Chinese cabbage fields. Morphological species differentiation between the two cyst nematodes is challenging, with only minor differences between them. Thus, this study described the newly intercepted
population, and reviewed morphological and molecular characteristics conceivably essential in differentiating the two nematode species. A comparison of morphometric measurements of both infective juveniles and vulval cones of cysts showed significant differences between the two cyst nematodes. When total RNA and genomic DNA were extracted from a mixed field population,
I genes and ITS regions were clearly amplified with primers of the two
species, suggesting that
population collected from the Chinese cabbage field consisted of a mixture of two species.
I and ITS of
were predominantly amplified from nucleotides prepared from
monoxenic population whereas those of
were strongly detected in
monoxenic cultures. Thus, this study confirms the coexistence of the two species in some Chinese cabbage fields; and the presence of
in Korea is reported here for the first time. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Handling Associate Editor: Lee, Yong Hoon These authors contributed equally to this work as first authors. |
ISSN: | 1598-2254 2093-9280 |
DOI: | 10.5423/PPJ.OA.12.2017.0262 |