Enhancement of HIV-1 DNA vaccine immunogenicity by BCG-PSN, a novel adjuvant

• Published in: Vaccine Vol. 31; no. 3; pp. 472 - 479
• Main Authors: Sun, Jing, Hou, Jue, Li, Dingfeng, Liu, Yong, Hu, Ningzhu, Hao, Yanling, Fu, Jingjing, Hu, Yunzhang, Shao, Yiming
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 07.01.2013; Elsevier; Elsevier Limited
• Subjects: Adjuvant; Adjuvants; Adjuvants, Immunologic - administration & dosage; Adjuvants, Immunologic - isolation & purification; AIDS Vaccines - administration & dosage; AIDS Vaccines - genetics; AIDS Vaccines - immunology; Allergy and Immunology; Animal models; Animals; Antigen presentation; antigens; Applied microbiology; BCG; BCG-PSN; Biological and medical sciences; China; Cytokines; Deoxyribonucleic acid; DNA; DNA vaccines; Enzymes; Female; Fundamental and applied biological sciences. Psychology; Gene expression; genes; HIV Antibodies - blood; HIV-1 - genetics; HIV-1 - immunology; HIV-1 DNA vaccine; Human immunodeficiency virus 1; humoral immunity; immune response; Immune response (humoral); Immune system; Immunization; Immunogenicity; Immunomodulation; Infectious diseases; Injections, Intramuscular; Laboratory animals; Medical research; Methods; Mice; Mice, Inbred BALB C; Microbiology; Miscellaneous; Mycobacterium bovis; Mycobacterium bovis - chemistry; Mycobacterium bovis BCG; nucleic acids; phenol; Phenols; Polysaccharides; Proteins; recombinant vaccines; secretion; Signal transduction; T-Lymphocytes - immunology; vaccination; Vaccination - methods; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, DNA - administration & dosage; Vaccines, DNA - genetics; Vaccines, DNA - immunology; Virology; China; IFN; IL; HIV-1 DNA vaccine; HIV; tumor necrosis factor; BCG-PSN; Adjuvant; interferon; interleukin; TNF; human immunodeficicency virus; Virus; Immunogenicity; HIV-1 virus; Immunological adjuvant; BCG; Retroviridae; Human immunodeficiency virus; Genetic vaccine; Lentivirus
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2012.11.024

Highlights ► We found that BCG-PSN could significantly improve the immunogenicity of DNA vaccine when co-administered with DNA vaccine. ► DNA vaccine co-administrated with BCG-PSN could decrease 10-fold dose of vaccine requirement. ► Our results demonstrate that BCG-PSN can activate TLR signaling pathways and induce Th1-type cytokines secretion. ► Our results may provide valuable information for designing an effective HIV-1 DNA vaccine immunization strategy.