Tissue-specific distribution of genistein, daidzein and bisphenol A in male Sprague-Dawley rats after intragastric administration

• Published in: Food Science and Technology Research Vol. 11; no. 2; pp. 187 - 193
• Main Authors: Yasuda, S, Wu, P.S, Okabe, M, Tachibana, H, Yamada, K
• Format: Journal Article
• Language: English
• Published: Tsukuba Japanese Society for Food Science and Technology 01.01.2005; Japan Science and Technology Agency
• Subjects: animal models; animal tissues; bisphenol A; daidzein; equol; genistein; intestinal absorption; intragastric administration; isoflavones; metabolism; metabolites; nutrient availability; plant estrogens; rats; uptake mechanisms
• ISSN: 1344-6606; 1881-3984
• DOI: 10.3136/fstr.11.187

A mixture of genistein, daidzein and bisphenol A was intragastrically given to male Sprague-Dawley rats. The tissue distribution of these compounds and equol, a metabolite of daidzein, was examined by high-performance liquid chromatography after administration. In the serum, liver and kidney, genistein, daidzein and bisphenol A were clearly detected at 6 to 24 h. Equol was detected at 0 h and levels subsequently dropped and then increased again, reaching the initial level at 24 h. In the mesenteric lymph node (MLN), thymus and spleen, genistein and daidzein were detected at 6 to 24 h. Bisphenol A and equol in the MLN was detectable at 6 to 24 h and 24 h, respectively. In the testicle, epididymis, spermatheca and prostate, higher levels of genistein and daidzein were detected around 6 h. Bisphenol A was detected in the epididymis at 6 to 12 h. These results suggest that the distribution of these chemicals and their tissue affinities in rats vary from tissue to tissue.