Circulating myeloid-derived suppressor cells in patients with pancreatic cancer

• Published in: Hepatobiliary & pancreatic diseases international Vol. 15; no. 1; pp. 099 - 105
• Main Authors: Xu, Xiao-Dong, Hu, Jun, Wang, Min, Peng, Feng, Tian, Rui, Guo, Xing-Jun, Xie, Yu, Qin, Ren-Yi
• Format: Journal Article
• Language: English
• Published: Singapore Elsevier B.V 01.02.2016
• Subjects: Aged; arginase; Arginase - blood; Bile Duct Neoplasms - blood; Bile Duct Neoplasms - immunology; Bile Duct Neoplasms - pathology; Biomarkers, Tumor - blood; Case-Control Studies; CD11b Antigen - blood; Cell Line, Tumor; Cholangiocarcinoma - blood; Cholangiocarcinoma - immunology; Cholangiocarcinoma - pathology; Coculture Techniques; Endocrinology & Metabolism; Female; Gastroenterology and Hepatology; GM-CSF; granulocyte-macrophage colony-stimulating factor; Granulocyte-Macrophage Colony-Stimulating Factor - blood; HLA-DR Antigens - blood; Humans; Leukocytes, Mononuclear - immunology; Leukocytes, Mononuclear - metabolism; Lipopolysaccharide Receptors - blood; Male; Middle Aged; Myeloid Cells - immunology; Myeloid Cells - metabolism; myeloid-derived suppressor cells; pancreatic cancer; Pancreatic Neoplasms - blood; Pancreatic Neoplasms - immunology; Pancreatic Neoplasms - pathology; Tumor Burden; Tumor Escape; 外周血单个核细胞; 循环; 癌症患者; 粒细胞-巨噬细胞集落刺激因子; 细胞类型; 胰腺癌; 酶联免疫吸附测定法; granulocyte-macrophage colony-stimulating factor; pancreatic cancer; myeloid-derived suppressor cells; arginase; granulocyte-macrophagecolony-stimulatingfactor
• ISSN: 1499-3872
• DOI: 10.1016/S1499-3872(15)60413-1

BACKGROUND： Myeloid-derived suppressor cells （MDSCs） are heterogeneous cell types that suppress T-cell responses in cancer patients and animal models, some MDSC subpopulations are increased in patients with pancreatic cancer. The present study was to investigate a specific subset of MDSCs in patients with pancreatic cancer and the mechanism of MDSCs increase in these patients. METHODS： Myeloid cells from whole blood were collected from 37 patients with pancreatic cancer, 17 with cholangiocarcinoma, and 47 healthy controls. Four pancreatic cancer cell lines were co- cultured with normal peripheral blood mononudear cells （PBMCs） to test the effect of tumor cells on the conversion of PBMCs to MDSCs. Levels of granulocyte-macrophage colony-stimulating factor （GM-CSF） and arginase activity in the plasma of cancer patients were analyzed by enzyme-linked immunosorbent assay. RESULTS： CD14＋/CD11b＋/HLA-DR MDSCs were increased in patients with pancreatic or bile duct cancer compared with those in healthy controls, and this increase was correlated with clinical cancer stage. Pancreatic cancer cell lines induced PBMCs to MDSCs in a dose-dependent manner. GM-CSF and arginase activity levels were significantly increased in the se rum of patients with pancreatic cancer. CONCLUSIONS： MDSCs were tumor related： tumor cells induced PBMCs to MDSCs in a dose-dependent manner and circulating CD14＋/CD11b＋/HLA-DR- MDSCs in pancreatic cancer patients were positively correlated with tumor burden. MDSCs might be useful markers for pancreatic cancer detection and progression.