Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link

• Published in: Food chemistry. Molecular sciences Vol. 5; p. 100144
• Main Authors: Verônica Cardoso de Souza, Bárbara, de Morais Sousa, Mariana, Augusto Gasparotto Sattler, José, Cristina Sousa Gramoza Vilarinho Santana, Ana, Bruno Fonseca de Carvalho, Rusbene, de Sousa Lima Neto, José, de Matos Borges, Fernando, Angelica Neri Numa, Iramaia, Braga Ribeiro, Alessandra, César Cunha Nunes, Lívio
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 30.12.2022; Elsevier
• Subjects: Bioactive compounds; ORAC; Phenolic compounds; Simulated digestion; Spray drying; ORAC; Spray drying; Phenolic compounds; Bioactive compounds; Simulated digestion
• ISSN: 2666-5662; 2666-5662
• DOI: 10.1016/j.fochms.2022.100144

•Polyphenols rich extracts from B. forficata were nanoencapsulated by spray drying.•The bioactive compounds of extracts were characterized by HPLC-PDA-ESI-IT-MS.•In vitro bioacessibility of bioactive compounds was evaluated.•Nanoencapsulated extracts after digestion maintained antioxidant activity.•Nanoencapsulated extracts are promisor candidate for nutraceuticals applications. Bauhinia forficata Link is a plant rich in polyphenols that has been used mainly for its hypoglycemic activity, which is related to its antioxidant and anti-inflammatory potential. However, the beneficial effect of these bioactive compounds is directly dependent on their bioaccessibility and bioavailability, requiring processing techniques that can improve and preserve their biological activities. This work aimed to obtain nanocapsulated extracts from the infusion (ESIN) and decoction (ESDC) of B. forficata Link leaves, by spray drying. The encapsulating agents used were maltodextrin and colloidal silicon dioxide. The nanocapsules were characterized by HPLC-PDA-ESI-IT-MSn, evaluated the bioaccessibility of polyphenols after simulated digestion and their antioxidant activity. Additionally, an extensive physicochemical characterization of the nanocapsulated extracts was carried out and their stability and technological parameters were evaluated. The ESIN and ESDC extracts had yields of 57.3 % and 62.7 %, with average nanocapsules sizes of 0.202 μm and 0.179 μm, low humidity and water activity (<0.5), powder density and proper flow properties (Hausner ratio ≤ 1.25; Carr index 18–19 %). Scanning electron microscopy showed a spherical and amorphous morphology and low viscosity, which may have favored the solubility profile. The phenolic compounds of the nanocapsules degraded after 400 °C, showing high thermal stability. The infrared spectra identified the presence of maltodextrin and phenolic compounds and that there were no reactions between them. Chromatography confirmed the presence of phenolic compounds, mainly flavonols and their O-glycosylated derivatives, as well as carbohydrates, probably maltodextrin. Simulated in vitro digestion showed that polyphenols and flavonoids from ESIN and ESDC nanocapsules were bioaccessible after the gastric phase (49.38 % and 64.17 % of polyphenols and 64.08 % and 36.61 % of flavonoids) and duodenal (52.68 % and 79.06 % of polyphenols and 13.24 % and 139.03 % of flavoids), with a variation from 52.27 % to 70.55 % of the antioxidant activity maintained, by the ORAC method, after gastric digestion and still 25 %, after duodenal. Therefore, the nanoencapsulation of extracts of B. forficata is a viable option for the preservation of their bioactive compounds, making them bioaccessible and with antioxidant activity, which make them suitable for incorporation into various nutraceutical formulations, such as capsules, tablets and sachets.