Multipotent mesenchymal stromal cells are fully permissive for human cytomegalovirus infection

• Published in: Virologica Sinica Vol. 31; no. 3; pp. 219 - 228
• Main Authors: Qiao, Guan-Hua, Zhao, Fei, Cheng, Shuang, Luo, Min-Hua
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.06.2016
• Subjects: abnormal development; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cells, Cultured; congenital abnormalities; Cytomegalovirus - genetics; Cytomegalovirus - physiology; Cytomegalovirus Infections - virology; fetal development; fluorescent antibody technique; Genome, Viral; HCMV; Herpesviridae; Human betaherpesvirus 5; Human cytomegalovirus; Humans; Medical Microbiology; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - virology; Microbial Genetics and Genomics; Microbiology; neonates; Oncology; pathogenesis; protein synthesis; Research Article; stem cells; stromal cells; umbilical cord; Umbilical Cord - cytology; Umbilical Cord - virology; Viral Plaque Assay; Viral Proteins - biosynthesis; Viral Proteins - genetics; Viral Proteins - metabolism; virion; Virology; Virus Internalization; Virus Replication; Western blotting; 人类; 免疫荧光检测; 器官形成; 巨细胞病毒; 病毒感染; 造血干/祖细胞; 骨髓间充质干细胞; Wharton’s jelly; multipotent mesenchymal stromal cells (MSCs); susceptibility; umbilical cord; human cytomegalovirus (HCMV)
• ISSN: 1674-0769; 1995-820X
• DOI: 10.1007/s12250-016-3754-0

Congenital human cytomegalovirus（HCMV） infection is a leading infectious cause of birth defects.Previous studies have reported birth defects with multiple organ maldevelopment in congenital HCMV-infected neonates. Multipotent mesenchymal stromal cells（MSCs） are a group of stem/progenitor cells that are multi-potent and can self-renew, and they play a vital role in multiorgan formation. Whether MSCs are susceptible to HCMV infection is unclear. In this study, MSCs were isolated from Wharton＇s jelly of the human umbilical cord and identified by their plastic adherence, surface marker pattern, and differentiation capacity. Then, the MSCs were infected with the HCMV Towne strain, and infection status was assessed via determination of viral entry,replication initiation, viral protein expression, and infectious virion release using western blotting,immunofluorescence assays, and plaque forming assays. The results indicate that the isolated MSCs were fully permissive for HCMV infection and provide a preliminary basis for understanding the pathogenesis of HCMV infection in non-nervous system diseases, including multi-organ malformation during fetal development.