Establishment and characterization of a new human acinar cell carcinoma cell line, Faraz-ICR, from pancreas

• Published in: Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] Vol. 17; no. 2; pp. 303 - 309
• Main Authors: Rezaei, Marzieh, Hosseini, Sayyed Ahmad, Nikeghbalian, Saman, Ghaderi, Abbas
• Format: Journal Article
• Language: English
• Published: Switzerland Elsevier B.V 01.03.2017; Elsevier Limited
• Subjects: Acinar cell carcinoma; Adenocarcinoma; Amphotericin B; Aneuploidy; Angiogenesis; Antineoplastic drugs; Antitumor agents; Atmosphere; Biological activity; Carbon dioxide; Carcinoma, Acinar Cell; Cdc42 protein; Cell culture; Cell line; Cell Line, Tumor; Cell Migration Assays; Cell Proliferation; Chemotherapy; Children; Chromosomes; Circular dichroism; Computer programs; Deoxyribonucleic acid; DNA; Drug abuse; Endocrinology & Metabolism; Enzymes; Female; Filters; Flow Cytometry; Gastroenterology and Hepatology; Humans; Hybridization; Malignancy; Metastases; Metastasis; Middle Aged; Motility; Pancreatic cancer; Pancreatic Neoplasms; Proteins; Radiation therapy; Surgery; Tissue culture; Tumor cells; Acinar cell carcinoma; Pancreatic cancer; Cell line
• ISSN: 1424-3903; 1424-3911
• DOI: 10.1016/j.pan.2017.02.003

Abstract Objectives Basic research in the field of acinar cell carcinoma (ACC) as a rare neoplasm of the pancreas is dependent on the availability of pragmatic model such as new pancreatic cancer cell lines. Thus, establishment and characterization of new pancreatic cancer cell lines from ACC origin are deemed important. Methods Faraz-ICR cell line was derived from a 58-years old woman with pancreatic acinar cell carcinoma by the collagenase digestion protocol. We characterized the cell line by examining its morphology and cytostructural and functional profile. Results Faraz-ICR has a doubling time of 35 h and grows in soft agar with a colony-forming efficiency of 25%. The cell had nearly normal pattern of chromosomes in karyotype analysis and Comparative Genomic Hybridization (CGH) array analysis. Evaluation of cells by flowcytometry showed that Faraz-ICR is negative for Epcam and mesenchymal markers in different passages, and has epithelial nature. Immunofluorescence staining revealed that cells were strongly positive for vimentin, desmin, ezrin, s100, nestin and they were negative for pan cytokeratins, chromogranin and alpha smooth muscle actin. Conclusions We were able to establish a new pancreatic carcinoma cell line with partial aspects of Epithelial–mesenchymal transition and aggressiveness. This cell line might be suitable for studying various anticancer drugs and protein profile aiming to see any possible tumor associated marker for ACC.