High-content analysis of cancer genome DNA alterations

New technologies as well as concerted brute-force approaches have increased the content (number of genes) that can be characterized for genomic DNA alterations. Recent advances include the detection of activating point mutations in key kinase genes ( BRAF , EGFR , and PIK3CA ) in multiple cancer typ...

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Bibliographic Details
Published inCurrent opinion in genetics & development Vol. 18; no. 1; pp. 68 - 72
Main Authors Degenhardt, Yan Y, Wooster, Richard, McCombie, Richard W, Lucito, Robert, Powers, Scott
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.02.2008
Subjects
DNA Mutational Analysis
DNA, Neoplasm - chemistry
Epigenesis, Genetic
Gene Dosage
Genes, Neoplasm
Genome, Human
Humans
Medical Education
Neoplasms - genetics
Oncogene Proteins, Fusion - genetics
Translocation, Genetic
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Summary:New technologies as well as concerted brute-force approaches have increased the content (number of genes) that can be characterized for genomic DNA alterations. Recent advances include the detection of activating point mutations in key kinase genes ( BRAF , EGFR , and PIK3CA ) in multiple cancer types: preliminary insight into the entire repertoire of genes that can be mutated in cancer; the discovery of new oncogenes by high-resolution profiling of DNA copy number alterations; and the bioinformatic-driven discovery of oncogenic gene fusions. High-content promoter methylation detection systems have been used to discover additional methylated genes and have provided evidence for a stem cell origin for certain tumors. Some of these advances have had significant impact on the development and clinical testing of new therapeutics.
Bibliography:ObjectType-Article-1
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ISSN:0959-437X
1879-0380
DOI:10.1016/j.gde.2008.01.005