Effect of the administration of Lactobacillus paracasei subsp. paracasei NTU 101 on Peyer's patch-mediated mucosal immunity

• Published in: International immunopharmacology Vol. 10; no. 7; pp. 791 - 798
• Main Authors: Tsai, Yueh-Ting, Cheng, Po-Ching, Liao, Jiunn-Wang, Pan, Tzu-Ming
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.07.2010; Elsevier
• Subjects: Animals; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; B-Lymphocytes - microbiology; B-Lymphocytes - pathology; Biological and medical sciences; CD4-Positive T-Lymphocytes - immunology; CD4-Positive T-Lymphocytes - metabolism; CD4-Positive T-Lymphocytes - microbiology; CD4-Positive T-Lymphocytes - pathology; CD40 Ligand - biosynthesis; Cell Communication; Cells, Cultured; Cytokines - metabolism; Dendritic Cells - immunology; Dendritic Cells - metabolism; Dendritic Cells - microbiology; Dendritic Cells - pathology; Immunity, Mucosal; Immunoglobulin A - biosynthesis; Immunoglobulin A - blood; Immunoglobulin A - genetics; Immunomodulation; Lactobacillus; Lactobacillus - immunology; Lactobacillus paracasei; Leukocyte Common Antigens - biosynthesis; Male; Medical sciences; Mice; Mice, Inbred BALB C; Mucosal immunity; Mucous Membrane - immunology; Mucous Membrane - microbiology; Mucous Membrane - pathology; Peyer's patches; Peyer's Patches - immunology; Peyer's Patches - microbiology; Peyer's Patches - pathology; Pharmacology. Drug treatments; Probiotics; Peyer's patches; Probiotics; Mucosal immunity; Immunomodulation; Probiotic; Local immunity; Peyer patch; Health food; Mucosa; Bacteria; Lactobacillaceae; Lactobacillus
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2010.04.012

The role of lactic acid bacteria in gut mucosal immunity was investigated by comparing the enhanced effects in the Peyer's patches and spleen of BALB/c mice fed daily with Lactobacillus paracasei subsp. paracasei NTU 101 for 3 to 9 weeks. After feeding with Lactobacillus, the percentage of CD4 + T cells in both Peyer's patches and the spleen was significantly increased; however, expression of CD 154 molecules, which play a pivotal role in cell-to-cell communication, on CD4 + T cells and the percentage of B220 + B cells increased only in Peyer's patches. Compared with systemic serum IgA, Peyer's patch-derived immunomodulation induced higher levels of intestinal IgA +-producing cells in the lamina propria. Our data also showed that feeding with Lactobacillus induced stronger CD4 + T cell-dendritic cell interaction, enhanced CD4 + T cell and B cell proliferation, and increased IL-1β, IL-10, IL-12, IFN-γ, and TNF-α mRNA expression in Peyer's patches, but not in the spleen. Here, we demonstrate that following Lactobacillus treatment, Peyer's patches exhibited a more distinct capacity to induce CD4 + T cell-dendritic cell interactions, lymphocyte proliferation, and cytokine secretion than the spleen, and thereby promoted greater intestinal IgA production that could enhance immunosurveillance to prevent intestinal infections or other intestinal pathologies.