Global methylation screening in the Arabidopsis thaliana and Mus musculus genome: applications of virtual image restriction landmark genomic scanning (Vi‐RLGS)
Understanding the role of ‘epigenetic’ changes such as DNA methylation and chromatin remodeling has now become critical in understanding many biological processes. In order to delineate the global methylation pattern in a given genomic DNA, computer software has been developed to create a virtual im...
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Published in | Nucleic acids research Vol. 31; no. 15; pp. 4490 - 4496 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Oxford University Press
01.08.2003
Oxford Publishing Limited (England) |
Subjects | |
Online Access | Get full text |
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Summary: | Understanding the role of ‘epigenetic’ changes such as DNA methylation and chromatin remodeling has now become critical in understanding many biological processes. In order to delineate the global methylation pattern in a given genomic DNA, computer software has been developed to create a virtual image of restriction landmark genomic scanning (Vi‐RLGS). When using a methylation‐ sensitive enzyme such as NotI as the restriction landmark, the comparison between real and in silico RLGS profiles of the genome provides a methylation map of genomic NotI sites. A methylation map of the Arabidopsis genome was created that could be confirmed by a methylation‐sensitive PCR assay. The method has also been applied to the mouse genome. Although a complete methylation map has not been completed, a region of methylation difference between two tissues has been tested and confirmed by bisulfite sequencing. Vi‐RLGS in conjunction with real RLGS will make it possible to develop a more complete map of genomic sites that are methylated or demethylated as a consequence of normal or abnormal development. |
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Bibliography: | ark:/67375/HXZ-5SG1S3X9-M istex:0B8CA295C5C99D9411279BB9C99B45A899FB0432 Received April 8, 2003; Revised and Accepted May 27, 2003 To whom correspondence should be addressed. Tel: +1 716 845 1546; Fax: +1 716 845 1698; Email: hiroki.nagase@roswellpark.org Correspondence may also be addressed to Shigeo Yoshida. Tel: +81 48 462 4674; Fax: +81 48 467 9524; Email: yshigeo@postman.riken.go.jp local:gkg488 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 To whom correspondence should be addressed. Tel: +1 716 845 1546; Fax: +1 716 845 1698; Email: hiroki.nagase@roswellpark.org Correspondence may also be addressed to Shigeo Yoshida. Tel: +81 48 462 4674; Fax: +81 48 467 9524; Email: yshigeo@postman.riken.go.jp |
ISSN: | 0305-1048 1362-4962 1362-4962 |
DOI: | 10.1093/nar/gkg488 |