A Biotin-PEAC5-maleimide labeling assay to detect electrophiles

Recently, we established a biotin-PEAC5-maleimide (BPM)-labeling assay, which can be used to determine the modification of electrophilic metals to proteins (Toyama et al., J. Toxicol. Sci., 38, 477-484, 2013). In the present study, we applied a BPM-labeling assay to detect protein S-modification by...

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Published inJournal of toxicological sciences Vol. 40; no. 3; pp. 405 - 411
Main Authors Abiko, Yumi, Luong, Nho Cong, Kumagai, Yoshito
Format Journal Article
LanguageEnglish
Published Japan The Japanese Society of Toxicology 2015
Japan Science and Technology Agency
Subjects
Animals
Biotin
Biotin-PEAC5-maleimide
Blotting, Western - methods
Cysteine
Cytosol - metabolism
Electrophile
Enzyme-Linked Immunosorbent Assay - methods
Humans
Liver - metabolism
Maleimides
Metals - chemistry
Mice
Naphthoquinones
Oxidation-Reduction
Proteins - analysis
Proteins - chemistry
Quinones - analysis
S-arylation
Signal Transduction
Thiol
Tumor Cells, Cultured
Volatilization
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Summary:Recently, we established a biotin-PEAC5-maleimide (BPM)-labeling assay, which can be used to determine the modification of electrophilic metals to proteins (Toyama et al., J. Toxicol. Sci., 38, 477-484, 2013). In the present study, we applied a BPM-labeling assay to detect protein S-modification by environmental organic electrophiles. After exposing A431 cells to 1,2-naphthoquinone (1,2-NQ) and 1,4-naphthoquinone (1,4-NQ), there was an inverse correlation between Western blot analysis with specific antibody against these electrophiles and that with BPM on the blot intensity to detect protein modification. Similar results were also observed using enzyme-linked immunosorbent assay (ELISA) with BPM. Modification of proteins in mouse liver cytosol by 5-hydroxy-1,4-NQ, 5,8-dihydroxy-1,4-NQ, 1,4-benzoquinone (1,4-BQ), tert-butyl-1,4-BQ, and N-acetyl-p-benzoquinoneimine, an electrophilic metabolite of acetaminophen, was detected using ELISA, but not non-electrophilic quinones or hydroquinone. We also tested whether ELISA could be used to detect electrophiles contained in the vapor phase of ambient air samples collected in the midtown area of Los Angeles. Taken together, the results suggested that the ELISA, developed in this study, can detect the existence of electrophilic quinones that covalently modify cellular proteins, resulting in modulation of redox-signal transduction pathways or cell damage.
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ISSN:0388-1350
1880-3989
DOI:10.2131/jts.40.405