Molecular Phylogeny of Mobatviruses ( Hantaviridae ) in Myanmar and Vietnam

• Published in: Viruses Vol. 11; no. 3; p. 228
• Main Authors: Arai, Satoru, Kikuchi, Fuka, Bawm, Saw, Sơn, Nguyễn Trường, Lin, Kyaw San, Tú, Vương Tân, Aoki, Keita, Tsuchiya, Kimiyuki, Tanaka-Taya, Keiko, Morikawa, Shigeru, Oishi, Kazunori, Yanagihara, Richard
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07.03.2019; MDPI
• Subjects: Amino acid sequence; Animals; Annealing; Bats; Bayes Theorem; Bayesian analysis; Chiroptera; Chiroptera - virology; Cytochrome; Disease Reservoirs - virology; Divergence; Enzymes; Genetic Variation; Genomes; Hantaviridae; Hantavirus; Hantavirus - classification; Hantavirus Infections - epidemiology; Hipposideros; Lung - virology; Markov analysis; Mitochondrial DNA; Mobatvirus; Myanmar - epidemiology; Phylogenetics; Phylogeny; RNA Viruses - genetics; RNA, Viral - genetics; Sequence Analysis, DNA; Species; Strains (organisms); Taphozous melanopogon; Vietnam - epidemiology; Myanmar; Vietnam; Myanmar (Burma); United States > US; China; Japan; Hantaviridae; phylogeny; Mobatvirus
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v11030228

The discovery of highly divergent lineages of hantaviruses (family ) in shrews, moles, and bats of multiple species raises the possibility that non-rodent hosts may have played a significant role in their evolutionary history. To further investigate this prospect, total RNA was extracted from RNAlater -preserved lung tissues of 277 bats (representing five families, 14 genera and 40 species), captured in Myanmar and Vietnam during 2013⁻2016. Hantavirus RNA was detected in two of 15 black-bearded tomb bats ( ) and two of 26 Pomona roundleaf bats ( ) in Myanmar, and in three of six ashy leaf-nosed bats ( ) in Vietnam. Pair-wise alignment and comparison of coding regions of the S, M, and L segments of hantaviruses from and bats revealed high nucleotide and amino acid sequence similarities to prototype Láibīn virus (LAIV) and Xuân Sơn virus (XSV), respectively. Phylogenetic analyses, generated by maximum-likelihood and Bayesian methods, showed a geographic clustering of LAIV strains from China and Myanmar, but not of XSV strains from China and Vietnam. These findings confirm that the black-bearded tomb bat is the natural reservoir of LAIV, and that more than one species of bats can host XSV.