Multiparameter flow cytometry quantification of bone marrow plasma cells at diagnosis provides more prognostic information than morphological assessment in myeloma patients

• Published in: Haematologica (Roma) Vol. 94; no. 11; pp. 1599 - 1602
• Main Authors: PAIVA, Bruno, VIDRIALES, Maria-Belén, PEREZ, Jose J, MATEO, Gema, MONTALBAN, Maria Angeles, MATEOS, Maria Victoria, BLADE, Joan, LAHUERTA, Juan José, ORFAO, Alberto, SAN MIGUEL, Jesús F
• Format: Journal Article
• Language: English
• Published: Pavia Ferrata Storti Foundation 01.11.2009
• Subjects: Adult; Aged; Biological and medical sciences; Bone Marrow Cells - pathology; Brief Reports; Cell Count; Diagnostic Techniques and Procedures; Female; Flow Cytometry - methods; Hematologic and hematopoietic diseases; Humans; Immunodeficiencies. Immunoglobulinopathies; Immunoglobulinopathies; Immunopathology; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Male; Medical sciences; Middle Aged; Multiple Myeloma - diagnosis; Multiple Myeloma - pathology; Prognosis; Human; multiparameter flow cytometry quantification; Immunopathology; Flow cytometry; multiple myeloma; Prognosis; Hematology; Immunophenotype; conventional morphology; B cell neoplasm; Malignant hemopathy; Plasmocyte; Lymphoid neoplasm; Information; Myeloma; Immunoglobulinopathy; immunophenotyping; Lymphoproliferative syndrome; Morphology; Bone marrow; Diagnosis; Cancer; Quantitative analysis
• ISSN: 0390-6078; 1592-8721
• DOI: 10.3324/haematol.2009.009100

Quantification of bone marrow plasma cells in multiple myeloma patients using conventional morphology is of limited prognostic value, while the merit of multiparameter flow cytometry immunophenotyping is still considered unproven. Here we compare the bone marrow plasma cell counts obtained by morphology and multiparameter flow cytometry and explore the potential prognostic impact of both techniques in 765 newly diagnosed, uniformly treated multiple myeloma patients. Although multiparameter flow cytometry generally yields lower plasma cell counts (median percentage of 11% vs. 40%, respectively; p<0.001), there is a significant positive correlation between the two techniques (R =0.46, p<0.001). Regarding prognosis, multivariate analysis selected the bone marrow plasma cell counts obtained by multiparameter flow cytometry as an independent prognostic factor for overall survival (p=0.007), supporting the incorporation of multiparameter flow cytometry immunophenotyping into the routine diagnostic evaluation of multiple myeloma patients and validating the clinical utility of bone marrow plasma cell counting by multiparameter flow cytometry approaches. (clinicaltrials.gov identifier: NCT00560053).