Characterization of Sclerotinia sclerotiorum, an Emerging Fungal Pathogen Causing Blight in Hyacinth Bean (Lablab purpureus)

• Published in: The plant pathology journal Vol. 34; no. 5; pp. 367 - 380
• Main Authors: Prova, Ananya, Akanda, Abdul Mannan, Islam, Shaikhul, Hossain, Md Motaher
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국식물병리학회 01.10.2018; Korean Society of Plant Pathology; Hanrimwon Publishing Company
• Subjects: Fungicides; host resistance; mycelial growth; new pathogen; sclerotial development; 농학; Fungicides; sclerotial development; new pathogen; mycelial growth; host resistance
• ISSN: 1598-2254; 2093-9280
• DOI: 10.5423/PPJ.OA.02.2018.0028

Stems and pods of hyacinth bean cultivated in a farmer's field in Gazipur District, Bangladesh, were found rotted in nearly 5% hyacinth bean plants. A fungus having fluffy mycelium and large sclerotia was isolated from affected tissues. Combined results of morphological, molecular and pathological analyses identified the fungus as (Lib) de Bary. Inoculating the fungus on healthy hyacinth bean plants and pods reproduced the symptoms previously observed in the field. The three isolates obtained from naturally infected plants were cross inoculated in hyacinth bean, okra and African-American marigold and they were pathogenic to these hosts. The optimum temperature and pH for its growth were 20°C and pH 5.0, respectively. Sclerotial development was favored at pH 5.0. Sucrose and mannitol were the best carbon sources to support hyphal growth, while glucose was the most favourable for sclerotial development. The hyacinth bean genotypes, HB-82 (Rupban Sheem) and HB-102 were found highly resistant, while HB-94 (Ashina) was moderate resistant to the fungus. Finally, was sensitive to Bavistin, Dithane M-45 and Rovral fungicides and Ca in the form of CaCl . This observation could possibly aid in eliminating field loss in hyacinth bean caused by an emerging pathogenic fungus .