Bile Tolerance, Taurocholate Deconjugation, and Binding of Cholesterol by Lactobacillus gasseri Strains

• Published in: Journal of dairy science Vol. 82; no. 2; pp. 243 - 248
• Main Authors: Usman, Hosono, A
• Format: Journal Article
• Language: English
• Published: Savoy, IL Elsevier Inc 01.02.1999; Am Dairy Sci Assoc; American Dairy Science Association
• Subjects: Bacteriology; Bile - physiology; bile salts; bile tolerance; binding; Biological and medical sciences; cholesterol; Cholesterol - metabolism; cholesterol-binding ability; Culture Media; Fundamental and applied biological sciences. Psychology; growth rate; Growth, nutrition, cell differenciation; Lactobacillus; Lactobacillus - growth & development; Lactobacillus - metabolism; Microbiology; probiotics; sodium taurocholate deconjugation; Species Specificity; strain differences; taurocholic acid; Taurocholic Acid - metabolism; LT; cholesterol-binding ability; sodium taurocholate deconjugation; bile tolerance; Probiotic; Microorganism growth; Bile salt; Environmental factor; Strain specificity; In vitro; Cholesterol; Binding capacity; Correlation analysis; Microorganism culture; Bacteria; Lactobacillaceae; Lactobacillus gasseri
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.S0022-0302(99)75229-X

Bile tolerance, deconjugation of sodium taurocholate, and the cholesterol-binding ability of 28 strains of Lactobacillus gasseri were examined. There was significant variation among strains in growth in media containing bile and also variation in the ability to bind cholesterol. Cultures grown for 12h at 37°C bound significantly more cholesterol than did cells from a 48-h incubation. Variation among strains in the ability to deconjugate sodium taurocholate was not significantly different. Maximal deconjugation of sodium taurocholate was achieved with the cells during the stationary phase of growth (12h). Statistical analysis showed no significant correlation between bile tolerance and sodium taurocholate deconjugation, bile tolerance and cholesterol-binding ability, or sodium taurocholate deconjugation and cholesterol-binding ability.