Molecular Determinants for Antibody Binding on Group 1 House Dust Mite Allergens

• Published in: The Journal of biological chemistry Vol. 287; no. 10; pp. 7388 - 7398
• Main Authors: Chruszcz, Maksymilian, Pomés, Anna, Glesner, Jill, Vailes, Lisa D., Osinski, Tomasz, Porebski, Przemyslaw J., Majorek, Karolina A., Heymann, Peter W., Platts-Mills, Thomas A.E., Minor, Wladek, Chapman, Martin D.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 02.03.2012; American Society for Biochemistry and Molecular Biology
• Subjects: 60 APPLIED LIFE SCIENCES; Allergens - chemistry; Allergens - genetics; Allergens - immunology; ALLERGY; AMINO ACID SEQUENCE; Animals; ANTIBODIES; Antibodies, Monoclonal, Murine-Derived - chemistry; Antibodies, Monoclonal, Murine-Derived - genetics; Antibodies, Monoclonal, Murine-Derived - immunology; Antigens, Dermatophagoides - chemistry; Antigens, Dermatophagoides - genetics; Antigens, Dermatophagoides - immunology; Arthropod Proteins - chemistry; Arthropod Proteins - genetics; Arthropod Proteins - immunology; ASTHMA; BASIC BIOLOGICAL SCIENCES; BIOLOGY; CRYSTAL STRUCTURE; CYSTEINE; Cysteine Endopeptidases - chemistry; Cysteine Endopeptidases - genetics; Cysteine Endopeptidases - immunology; Der f 1; Der p1; Dust Mites; DUSTS; Epitopes - chemistry; Epitopes - genetics; Epitopes - immunology; Immunoglobulin E - chemistry; Immunoglobulin E - genetics; Immunoglobulin E - immunology; IMMUNOLOGY; Mice; MITES; Molecular Bases of Disease; Mutation; MUTATIONS; Pyroglyphidae; RESIDUES; Structural Biology; TARGETS; VACCINES; Vaccines - chemistry; Vaccines - genetics; Vaccines - immunology; Allergy; Immunology; Dust Mites; Structural Biology; Antibodies; Der p1; Asthma; Der f 1
• ISSN: 0021-9258; 1083-351X; 1083-351X
• DOI: 10.1074/jbc.M111.311159

House dust mites produce potent allergens, Der p 1 and Der f 1, that cause allergic sensitization and asthma. Der p 1 and Der f 1 are cysteine proteases that elicit IgE responses in 80% of mite-allergic subjects and have proinflammatory properties. Their antigenic structure is unknown. Here, we present crystal structures of natural Der p 1 and Der f 1 in complex with a monoclonal antibody, 4C1, which binds to a unique cross-reactive epitope on both allergens associated with IgE recognition. The 4C1 epitope is formed by almost identical amino acid sequences and contact residues. Mutations of the contact residues abrogate mAb 4C1 binding and reduce IgE antibody binding. These surface-exposed residues are molecular targets that can be exploited for development of recombinant allergen vaccines. A unique, cross-reacting monoclonal antibody binds both Der f 1 and Der p 1. A common epitope present on both Der f 1 and Der p 1 was identified and mutated. Mutagenesis and antibody binding analysis allowed identification of IgE antibody binding sites. The obtained data will lead to the production of hypoallergens with low IgE antibody binding capacity.