Crystallographic structure of a small molecule SIRT1 activator-enzyme complex

• Published in: Nature communications Vol. 6; no. 1; p. 7645
• Main Authors: Dai, Han, Case, April W., Riera, Thomas V., Considine, Thomas, Lee, Jessica E., Hamuro, Yoshitomo, Zhao, Huizhen, Jiang, Yong, Sweitzer, Sharon M., Pietrak, Beth, Schwartz, Benjamin, Blum, Charles A., Disch, Jeremy S., Caldwell, Richard, Szczepankiewicz, Bruce, Oalmann, Christopher, Yee Ng, Pui, White, Brian H., Casaubon, Rebecca, Narayan, Radha, Koppetsch, Karsten, Bourbonais, Francis, Wu, Bo, Wang, Junfeng, Qian, Dongming, Jiang, Fan, Mao, Cheney, Wang, Minghui, Hu, Erding, Wu, Joe C., Perni, Robert B., Vlasuk, George P., Ellis, James L.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 02.07.2015; Nature Publishing Group; Nature Pub. Group
• Subjects: 631/154/309; 631/45/173; 631/45/535/1266; 631/57/2272; 82; Amino Acid Sequence; Amino acids; Binding sites; Binding Sites - genetics; Catalytic Domain - genetics; Crystallization; Crystallography, X-Ray; Deuterium Exchange Measurement; Enzymes; Escherichia coli; Genetic Vectors; Humanities and Social Sciences; Humans; Lysine - metabolism; Mass Spectrometry; Molecular Dynamics Simulation; multidisciplinary; Mutagenesis; Mutagenesis, Site-Directed; Peptides; Protein Binding; Proteins; Science; Science (multidisciplinary); Scientific imaging; Sirtuin 1 - chemistry; Sirtuin 1 - genetics; Sirtuin 1 - metabolism; Transfection; United States > US; China
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/ncomms8645

SIRT1, the founding member of the mammalian family of seven NAD + -dependent sirtuins, is composed of 747 amino acids forming a catalytic domain and extended N- and C-terminal regions. We report the design and characterization of an engineered human SIRT1 construct (mini-hSIRT1) containing the minimal structural elements required for lysine deacetylation and catalytic activation by small molecule sirtuin-activating compounds (STACs). Using this construct, we solved the crystal structure of a mini-hSIRT1-STAC complex, which revealed the STAC-binding site within the N-terminal domain of hSIRT1. Together with hydrogen-deuterium exchange mass spectrometry (HDX-MS) and site-directed mutagenesis using full-length hSIRT1, these data establish a specific STAC-binding site and identify key intermolecular interactions with hSIRT1. The determination of the interface governing the binding of STACs with human SIRT1 facilitates greater understanding of STAC activation of this enzyme, which holds significant promise as a therapeutic target for multiple human diseases. Sirtuins are NAD + -dependent deacylases implicated in the regulation of stress responses, bioenergetics and epigenetic control. Here the authors describe the crystal structure of a sirtuin-activating compounds (STAC)-sirtuin complex and begin to elucidate the mechanism of sirtuins activation by STACs.