Enhanced Production of Citric Acid in Yarrowia lipolytica by Triton X-100

• Published in: Applied biochemistry and biotechnology Vol. 165; no. 3-4; pp. 1068 - 1074
• Main Authors: Mirbagheri, Maryam, Nahvi, Iraj, Emtiazi, Giti, Darvishi, Farshad
• Format: Journal Article
• Language: English
• Published: New York Humana Press Inc 01.10.2011; Springer; Springer Nature B.V
• Subjects: Acids; Biochemistry; Biological and medical sciences; Biotechnology; Cell Membrane Permeability - drug effects; Chemicals; Chemistry; Chemistry and Materials Science; Citric Acid - metabolism; Fundamental and applied biological sciences. Psychology; Glucose - metabolism; Industrial Microbiology - methods; Microscopy, Electron, Scanning; Octoxynol - chemistry; Octoxynol - pharmacology; Permeability; Surface-Active Agents - chemistry; Surface-Active Agents - pharmacology; Surfactants; Yarrowia - drug effects; Yarrowia - metabolism; Yarrowia lipolytica; Yeasts; Permeabilization; Surfactant; Citric acid; Triton X-100; Ascomycota; Fungi; Production; Permeabilizing; Yarrowia lipolytica
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-011-9325-9

Various chemical surfactants could affect permeability of yeast cells. In this study, effects of the surfactant addition upon yeast cells permeability and citric acid (CA) production by Yarrowia lipolytica strains DSM 3286 and M7 were investigated. The addition of Triton X-100 increased 1.4–1.8-fold of the maximum CA quantity achieved for both strains, with final CA concentrations ranging between 75–85 g/l that correspond to CA conversion yields per unit of glucose consumed of ~0.80–0.84 g/g. Scanning electron micrographs of yeast cells showed that the cells treated with Triton X-100 had altered cell structure and were smaller and narrower compared with the non-treated ones. The results showed that Triton X-100 could be used in order to increase the efficiency of CA production by Y. lipolytica strains.