Quantification of L-ergothioneine in whole blood by hydrophilic interaction ultra-performance liquid chromatography and UV-detection

• Published in: Journal of separation science Vol. 36; no. 6; pp. 1002 - 1006
• Main Authors: Sotgia, Salvatore, Zinellu, Angelo, Pintus, Gianfranco, Pinna, Gerard Aime, Deiana, Luca, Carru, Ciriaco
• Format: Journal Article
• Language: English
• Published: Weinheim Blackwell Publishing Ltd 01.03.2013; Wiley; Wiley Subscription Services, Inc
• Subjects: 1.7 μm Ethylene bridged hybrid particles; Aged; Aged, 80 and over; Ammonium acetate; Betaine; Biological and medical sciences; Blood; Chromatography; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Ergothioneine - blood; Female; Humans; Hydrophilic interaction chromatography; Hydrophobic and Hydrophilic Interactions; Investigative techniques, diagnostic techniques (general aspects); L-Ergothioneine; Linearity; Liquid chromatography; Male; Medical sciences; Miscellaneous. Technology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Recovery; Routines; Separation; Spectrophotometry, Ultraviolet; Thione; Ultraviolet radiation; Human; Ultra performance liquid chromatography; Biological fluid; 1.7 μm Ethylene bridged hybrid particles; Chemical analysis; L-Ergothioneine; Healthy subject; Hydrophilic Interaction Liquid chromatography; Sulfur containing aminoacid; Whole blood; Ultraviolet detector; Thione; Hydrophilic interaction chromatography; Betaine; Clinical biology; Betaines; Quantitative analysis
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.201201016

A new hydrophilic interaction ultra‐performance LC method was established for the whole blood measurement of L‐ergothioneine. Chromatographic separation was achieved in a fairly short time, less than 4 min, on a 100 × 2.1 mm Acquity UPLC BEH HILIC 1.7 μm column with a mobile phase consisting of a mixture of 100 mmol/L ammonium acetate/ACN/water (5:85:10, v/v/v) that flowed isocratically at 0.250 mL/min. The LOD and the limit of quantification were 3.85 and 11.67 μmol/L, respectively. The method exhibited linearity in a concentration range of 15.63–1000 μmol/L (R2 > 0.999). Mean recovery was 96.34% whereas intraassay and interassay precision were 1.52 and 1.82% RSD, respectively. On the whole, the developed method is simple, fast, precise, accurate, and sensitive and may be useful for routine analyses.