Mycobacterium avium ssp. hominissuis biofilm is composed of distinct phenotypes and influenced by the presence of antimicrobials

• Published in: Clinical microbiology and infection Vol. 17; no. 5; pp. 697 - 703
• Main Authors: McNabe, M., Tennant, R., Danelishvili, L., Young, L., Bermudez, L.E.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier Ltd 01.05.2011; Blackwell Publishing Ltd; Wiley-Blackwell; Elsevier Limited
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Antibacterial agents; Antibiotics; Antibiotics. Antiinfectious agents. Antiparasitic agents; biofilm; Biofilms - drug effects; Biological and medical sciences; Bronchiectasis - drug therapy; Bronchiectasis - microbiology; DNA-Binding Proteins; Drug Resistance, Bacterial; gene expression; Infectious diseases; Lung - microbiology; M. avium; Medical sciences; Mice; Mice, Inbred C57BL; Microbial Sensitivity Tests; Mycobacterium avium - drug effects; Mycobacterium avium - genetics; Mycobacterium avium - physiology; Pharmacology. Drug treatments; Phenotype; Polymerase Chain Reaction; M. avium; Antibiotics; gene expression; biofilm; avium; Mycobacterium avium; Gene expression; M; Antimicrobial agent; Infection; Antibiotic; Phenotype; Mycobacteriales; Mycobacteriaceae; Biofilm; Bacteria; Actinomycetes; Antibacterial agent
• ISSN: 1198-743X; 1469-0691
• DOI: 10.1111/j.1469-0691.2010.03307.x

Mycobacterium avium ssp. hominissuis, hereafter referred to as M. avium, forms biofilm, a property that, in mice, is associated with lung infection via aerosol. As M. avium might co‐inhabit the respiratory tract with other pathogens, treatment of the co‐pathogen‐associated infections, such as in bronchiectasis, would expose M. avium to therapeutic compounds that may have their origin in other organisms sharing the natural environments. Incubation of M. avium with two compounds produced by environmental organisms, streptomycin and tetracycline, in vitro at subinhibitory concentrations increased biofilm formation in a number of M. avium strains, although exposure to ampicillin, moxifloxacin, rifampin and trimethoprim–sulphamethoxazole had no effect on biofilm formation. No selection of genotypically resistant clones was observed. Although incubation of bacteria in the presence of streptomycin upregulates the expression of biofilm‐associated genes, the response to the antibiotics had no association with the expression of a regulator (LysR) linked to the formation of biofilm in M. avium. Biofilms are composed of planktonic and sessile bacteria. Whereas planktonic M. avium is susceptible to clarithromycin and ethambutol (clinically used antimicrobials), sessile bacteria are at least three‐fold to four‐fold more resistant to antibiotics. The sessile phenotype, however, is reversible, and no selection of resistant clones was observed. Mice infected through the airway with both phenotypes were infected with a similar number of bacteria, demonstrating no phenotype advantage. M. avium biofilm formation is enhanced by commonly used compounds and, in the sessile bacterial phenotype, is resistant to clarithromycin and ethambutol, in a reversible manner.