Molecular inventory of faecal microflora in patients with Crohn's disease

• Published in: FEMS microbiology ecology Vol. 50; no. 1; pp. 25 - 36
• Main Authors: Mangin, Irène, Bonnet, Régis, Seksik, Philippe, Rigottier-Gois, Lionel, Sutren, Malène, Bouhnik, Yoram, Neut, Christel, Collins, Matthew D., Colombel, Jean-Frédéric, Marteau, Philippe, Doré, Joël
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 01.10.2004; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: 16S rRNA gene sequencing; Abnormalities; Bacteria; Bacteria - classification; Bacteria - isolation & purification; Bacteriology; Bacteroides vulgatus; Biodiversity; Biological and medical sciences; Clostridium; Clostridium glycolicum; Clostridium perfringens; Clostridium ramosum; Cluster Analysis; Colon; Community involvement; Crohn Disease - microbiology; Crohn's disease; Disease control; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; E coli; Ecology; Escherichia coli; Faecal bacterial diversity; Feces - microbiology; Fundamental and applied biological sciences. Psychology; Fusobacterium; Humans; Intestine; Microbiology; Microflora; Microorganisms; Miscellaneous; Molecular Sequence Data; Nucleotide sequence; Pathogenesis; Pectinatus; Phylogeny; Ribosomal DNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Species; Sutterella; Verrucomicrobium; Faecal bacterial diversity; Crohn's disease; 16S rRNA gene sequencing; Fusobacterium; Pathogenesis; Escherichia coli; Ribosomal DNA; Clostridiales; Microflora; Clostridium innocuum; Phylogeny; Clostridium perfringens; Bacteroidaceae; Inflammatory disease; Gene; Ribosomal RNA; Bacteroides vulgatus; 16S-RNA; Bacteria; Intestinal disease; Microbial community; Enterobacteriaceae; Human; Crohn disease; Clostridiaceae; Digestive diseases; Clostridium ramosum

Intestinal microbial community is involved in the pathogenesis of Crohn's disease, but knowledge of its potential abnormalities has been limited by the impossibility to grow many dominant intestinal bacteria. Using sequence analysis of randomly cloned bacterial 16S ribosomal DNA, the dominant f...