Molecular inventory of faecal microflora in patients with Crohn's disease

• Published in: FEMS microbiology ecology Vol. 50; no. 1; pp. 25 - 36
• Main Authors: Mangin, Irène, Bonnet, Régis, Seksik, Philippe, Rigottier-Gois, Lionel, Sutren, Malène, Bouhnik, Yoram, Neut, Christel, Collins, Matthew D., Colombel, Jean-Frédéric, Marteau, Philippe, Doré, Joël
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 01.10.2004; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: 16S rRNA gene sequencing; Abnormalities; Bacteria; Bacteria - classification; Bacteria - isolation & purification; Bacteriology; Bacteroides vulgatus; Biodiversity; Biological and medical sciences; Clostridium; Clostridium glycolicum; Clostridium perfringens; Clostridium ramosum; Cluster Analysis; Colon; Community involvement; Crohn Disease - microbiology; Crohn's disease; Disease control; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; E coli; Ecology; Escherichia coli; Faecal bacterial diversity; Feces - microbiology; Fundamental and applied biological sciences. Psychology; Fusobacterium; Humans; Intestine; Microbiology; Microflora; Microorganisms; Miscellaneous; Molecular Sequence Data; Nucleotide sequence; Pathogenesis; Pectinatus; Phylogeny; Ribosomal DNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Species; Sutterella; Verrucomicrobium; Faecal bacterial diversity; Crohn's disease; 16S rRNA gene sequencing; Fusobacterium; Pathogenesis; Escherichia coli; Ribosomal DNA; Clostridiales; Microflora; Clostridium innocuum; Phylogeny; Clostridium perfringens; Bacteroidaceae; Inflammatory disease; Gene; Ribosomal RNA; Bacteroides vulgatus; 16S-RNA; Bacteria; Intestinal disease; Microbial community; Enterobacteriaceae; Human; Crohn disease; Clostridiaceae; Digestive diseases; Clostridium ramosum
• ISSN: 0168-6496; 1574-6941; 1574-6941
• DOI: 10.1016/j.femsec.2004.05.005

Intestinal microbial community is involved in the pathogenesis of Crohn's disease, but knowledge of its potential abnormalities has been limited by the impossibility to grow many dominant intestinal bacteria. Using sequence analysis of randomly cloned bacterial 16S ribosomal DNA, the dominant faecal species from four Crohn's disease patients and four controls were compared. Whereas marked inter-individual differences were observed in the faecal microflora of patients, three remained distantly related to controls on the basis of their operational taxonomic unit composition. Bacteroides vulgatus and closely related organisms represented the only molecular species shared by all patients and exhibited an unusually high rate of occurrence. Escherichia coli clones were isolated only in two patients with ileocolonic Crohn's disease. Moreover, numerous clones belonged to phylogenetic groups or species that are commonly not dominant in the faecal microflora of healthy subjects: Pectinatus, Sutterella, Verrucomicrobium, Fusobacterium, Clostridium disporicum, Clostridium glycolicum, Clostridium ramosum, Clostridium innocuum and Clostridium perfringens.