A single nucleotide polymorphism in the alternative oxidase gene among rice varieties differing in low temperature tolerance

Alternative oxidase (AOX) is encoded in a multigene family, and multiple isoforms have been observed in various plant species. We found for the first time an allelic variation in the same AOX locus. On SDS–gel blots of callus protein of rice (Oryza sativa L.), varieties without the QTL for low tempe...

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Bibliographic Details
Published inFEBS letters Vol. 527; no. 1-3; pp. 181 - 185
Main Authors Abe, Fumitaka, Saito, Koji, Miura, Kiyoyuki, Toriyama, Kinya
Format Journal Article
LanguageEnglish
Published England 11.09.2002
Subjects
Alternative oxidase
Asparagine - genetics
Chromosome Mapping
Genetic Variation
Isoforms
Low temperature tolerance
Lysine - genetics
Mitochondrial Proteins
Oryza - physiology
Oryza sativa L
Oxidoreductases - chemistry
Oxidoreductases - genetics
Plant Proteins - chemistry
Plant Proteins - genetics
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Quantitative Trait, Heritable
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Single nucleotide polymorphism
Temperature
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Summary:Alternative oxidase (AOX) is encoded in a multigene family, and multiple isoforms have been observed in various plant species. We found for the first time an allelic variation in the same AOX locus. On SDS–gel blots of callus protein of rice (Oryza sativa L.), varieties without the QTL for low temperature tolerance showed a 32‐kDa AOX band, whereas those with the QTL showed a 34‐kDa band. The variation was attributed to the substitution of Lys71 for Asn71 caused by a single nucleotide polymorphism between alleles of OsAOX1a, and was tightly linked to the presence of the QTL.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(02)03218-0