Rapid Induction of Distinct Stress Responses after the Release of Singlet Oxygen in Arabidopsis

The conditional fluorescent (flu) mutant of Arabidopsis accumulates the photosensitizer protochlorophyllide in the dark. After a dark-to-light shift, the generation of singlet oxygen, a nonradical reactive oxygen species, starts within the first minute of illumination and was shown to be confined to...

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Published inThe Plant cell Vol. 15; no. 10; pp. 2320 - 2332
Main Authors Roel G. L. op den Camp, Przybyla, Dominika, Ochsenbein, Christian, Laloi, Christophe, Kim, Chanhong, Danon, Antoine, Wagner, Daniela, Hideg, Éva, Göbel, Cornelia, Feussner, Ivo, Nater, Mena, Apel, Klaus
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Biologists 01.10.2003
American Society of Plant Biologists (ASPB)
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Summary:The conditional fluorescent (flu) mutant of Arabidopsis accumulates the photosensitizer protochlorophyllide in the dark. After a dark-to-light shift, the generation of singlet oxygen, a nonradical reactive oxygen species, starts within the first minute of illumination and was shown to be confined to plastids. Immediately after the shift, plants stopped growing and developed necrotic lesions. These early stress responses of the flu mutant do not seem to result merely from physicochemical damage. Peroxidation of chloroplast membrane lipids in these plants started rapidly and led to the transient and selective accumulation of a stereospecific and regiospecific and isomer of hydroxyoctadecatrieonic acid, free (13S)-HOTE, that could be attributed almost exclusively to the enzymatic oxidation of linolenic acid. Within the first 15 min of reillumination, distinct sets of genes were activated that were different from those induced by superoxide/hydrogen peroxide. Collectively, these results demonstrate that singlet oxygen does not act primarily as a toxin but rather as a signal that activates several stress-response pathways. Its biological activity in Arabidopsis exhibits a high degree of specificity that seems to be derived from the chemical identity of this reactive oxygen species and/or the intracellular location at which it is generated.
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PMCID: PMC197298
ISSN:1040-4651
1532-298X
1532-298X
DOI:10.1105/tpc.014662