A Straightforward Method for Adipocyte Size and Count Analysis Using Open-source Software QuPath

• Published in: Adipocyte Vol. 11; no. 1; pp. 99 - 107
• Main Authors: Palomäki, Ville A, Koivukangas, Vesa, Meriläinen, Sanna, Lehenkari, Petri, Karttunen, Tuomo J
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 31.12.2022; Taylor & Francis Group
• Subjects: Adipocyte size; Adipocyte Tools; Adipocytes - metabolism; Adipose Tissue - metabolism; Diabetes Mellitus, Type 2 - pathology; fat cell size; Humans; ImageJ; obesity; Obesity - metabolism; Qupath; Research Paper; Software; ImageJ; Adipocyte size; Adipocyte Tools; Qupath; fat cell size; obesity
• ISSN: 2162-3945; 2162-397X
• DOI: 10.1080/21623945.2022.2027610

Changes in adipose tissue morphology, depicted by cell morphology alterations such as enlargement of fat cells, always accompany over-weight and obesity. The variables related to cell size have been shown to associate with low-grade inflammation of adipose tissue and common obesity-related comorbidities including metabolic syndrome and type 2 diabetes. Quantifying fat cell morphology from images of histological specimens can be tedious. Here, we present a straightforward method for the task using the free open-source software QuPath with its inbuilt tools only. Measurements of human adipose tissue samples with the described protocol showed an excellent correlation with those obtained with ImageJ software with Adipocyte Tools plugin combined with manual correction of misdetections. Intraclass correlation between the two methods was at good to excellent level. The method described here can be applied to considerably large tissue areas, even whole-slide analysis.