Transcriptome Analysis of the Small Brown Planthopper, Laodelphax striatellus Carrying Rice stripe virus

• Published in: The plant pathology journal Vol. 29; no. 3; pp. 330 - 337
• Main Authors: Lee, Joo Hyun, Choi, Jae Young, Tao, Xue Ying, Kim, Jae Su, Kim, Woojin, Je, Yeon Ho
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국식물병리학회 01.09.2013; Korean Society of Plant Pathology; Hanrimwon Publishing Company
• Subjects: Laodelphax striatellus; Rice stripe virus; small brown planthopper; tenuivirus; transcriptome; 농학; Rice stripe virus; Laodelphax striatellus; tenuivirus; small brown planthopper; transcriptome
• ISSN: 1598-2254; 2093-9280
• DOI: 10.5423/PPJ.NT.01.2013.0001

Rice stripe virus (RSV), the type member of the genus Tenuivirus, transmits by the feeding behavior of small brown planthopper (SBPH), Laodelphax striatellus. To investigate the interactions between the virus and vector insect, total RNA was extracted from RSV-viruliferous SBPH (RVLS) and non-viruliferous SBPH (NVLS) adults to construct expressed sequence tag databases for comparative transcriptome analysis. Over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of isotigs from the mRNA of RVLS and NVLS, respectively. The gene ontology (GO) analysis demonstrated that both libraries have similar GO structures, however, the gene expression pattern analysis revealed that 17.8% and 16.8% of isotigs were up- and down-regulated significantly in the RVLS, respectively. These RSV-dependently regulated genes possibly have important roles in the physiology of SBPH, transmission of RSV, and RSV and SBPH interaction.