Inactivation of MyoD in mice leads to up-regulation of the myogenic HLH gene Myf-5 and results in apparently normal muscle development
The myogenic basic HLH transcription factor family of genes, composed of MyoD, myogenin, Myf-5, and Myf-6, are thought to regulate skeletal muscle differentiation. To understand the role of MyoD in myogenesis, we have introduced a null mutation of MyoD into the germline of mice. Surprisingly, mice l...
Saved in:
Published in | Cell Vol. 71; no. 3; pp. 383 - 390 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Cambridge, MA
Elsevier Inc
30.10.1992
Cell Press |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The myogenic basic HLH transcription factor family of genes, composed of
MyoD, myogenin,
Myf-5, and
Myf-6, are thought to regulate skeletal muscle differentiation. To understand the role of
MyoD in myogenesis, we have introduced a null mutation of
MyoD into the germline of mice. Surprisingly, mice lacking
MyoD are viable and fertile. Histological examination of skeletal muscle failed to reveal any morphological abnormalities in these mice. Furthermore, Northern analysis revealed normal levels of skeletal muscle-specific mRNAs. Significantly,
Myf-5 mRNA levels are elevated in postnatal mutant mice. Normally,
Myf-5 expression becomes markedly reduced at day 12 of gestation when
MyoD mRNA first appears. This suggests that
Myf-5 expression is repressed by
MyoD. Our results indicate that
MyoD is dispensable for skeletal muscle development in mice, revealing some degree of functional redundancy in the control of the skeletal myogenic developmental program. |
---|---|
Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0092-8674 1097-4172 |
DOI: | 10.1016/0092-8674(92)90508-A |