Gangliosides of human cerebrospinal fluid in various neurologic diseases
Simultaneous profile determination and quantification of human cerebrospinal fluid (CSF) gangliosides in various neurologic diseases ( n = 71) was examined. Gangliosides were extracted with methanol/chloroform from clinically available amounts of CSF (4–5 ml), then separated and quantified by high-p...
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Published in | Journal of the neurological sciences Vol. 105; no. 2; pp. 192 - 199 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
1991
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Subjects | |
Online Access | Get full text |
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Summary: | Simultaneous profile determination and quantification of human cerebrospinal fluid (CSF) gangliosides in various neurologic diseases (
n = 71) was examined. Gangliosides were extracted with methanol/chloroform from clinically available amounts of CSF (4–5 ml), then separated and quantified by high-performance thin-layer chromatography (HPTLC) and direct densitometry. Based on chromatographic comparison with standards, the percentage of lipid-bound NeuAc positive fractions in ‘normal’ CSF samples were: GM1 (II
3 NeuAc-GgOse
4Cer) (3%); GD3 (II
3 NeuAc
2-Lac-Cer) (4%); GD1a (IV
3 NeuAc, II
3 NeuAc-GgOse
4 Cer) (15%); X1 (3%); GD1b (II
3(NeuAc)
2-GgOse
4 Cer) (16%); X2 (4%); GT1b (IV
3 NeuAc, II
3(NeuAc)
2-GgOse
4-Cer) (40%); and GQ1b (IV
3(NeuAc)
2, II
3(NeuAc)
2 – GgOse
4-Cer (15%). Similarity between CSF and human cerebellar cortex, particularly in proportion of “b” series gangliosides (GQ1b, GT1b, GD1b), could be observed. A higher proportion of GD1a ganglioside, with decreased GQ1b was found in infancy. The total ganglioside content (mean ± 2 SD) varied between 645–894 μg/1. Significant alterations of the CSF ganglioside profile, with an increase in less polar gangliosides, GM3 and GD3, correlated with the blood-brain barrier dysfunction (CSF hemorrhages, compressive syndrome), or some malignant processes (metastatic brain melanoma). A statistically significant increase in the content of total CSF gangliosides was found in the following groups of patients as compared to controls: (1) ischemic cerebrovascular accident (CVI) with good outcome (
P < 0.02); (2) peripheral neuropathy and polyneuropathy (
P < 0.001) and (3) intravertebral discopathy (
P < 0.05). A significant decrease in the content of total CSF gangliosides was found in CVI group with lethal outcome (
P < 0.05). |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0022-510X 1878-5883 |
DOI: | 10.1016/0022-510X(91)90144-V |