Citricoccus yambaruensis sp. nov., a racemic phenylsuccinate stereospecifically assimilating actinomycete isolated from soil in Okinawa

A bacterial strain, designated PS9T, was isolated from soil in the Ryukyu Archipelago, Japan. The bacterium grew with racemic phenylsuccinate as the sole carbon and energy source. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain PS9T was closely related to Citricoccus mur...

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Published inJournal of general and applied microbiology Vol. 58; no. 5; pp. 373 - 378
Main Authors Matsui, Toru, Semba, Hisashi, Hanada, Satoshi
Format Journal Article
LanguageEnglish
Published Tokyo Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 2012
Microbiology Research Foundation
Subjects
actinomycetes
Biological and medical sciences
Citricoccus muralis
Citricoccus sp
Fatty Acids - analysis
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Microbiology
Micrococcaceae - classification
Micrococcaceae - genetics
Micrococcaceae - isolation & purification
Phenotype
phenylsuccinate
Phylogeny
Quinones - analysis
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
Soil Microbiology
Species Specificity
Stereoisomerism
Succinates - analysis
Citricoccus sp
Racemic
Soils
Bacteria
phenylsuccinate
Actinomycetes
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Summary:A bacterial strain, designated PS9T, was isolated from soil in the Ryukyu Archipelago, Japan. The bacterium grew with racemic phenylsuccinate as the sole carbon and energy source. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain PS9T was closely related to Citricoccus muralis, C. alkalitolerans and C. nitrophenolicus with sequence similarities of 97.5, 97.8, and 98.3%, respectively, suggesting that the strain belonged to the genus Citricoccus. Strain PS9T was a Gram-positive, non-motile, circular-shaped and aerobic bacterium. The major respiratory quinone was MK-8 (H2) and the predominant cellular fatty acid was C15:0 anteiso, C17:0 anteiso, and C15:0 iso. The G+C content was 72.4 mol%. Based on the phylogenetic and phenotypic traits, it was concluded that the organism represents a new species in the genus Citricoccus, with the name Citricoccus yambaruensis. The type strain is PS9T (=NBRC102121T = DSM18783T).
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ISSN:0022-1260
1349-8037
DOI:10.2323/jgam.58.373