Wound healing activity of Origanum vulgare engineered titanium dioxide nanoparticles in Wistar Albino rats

• Published in: Journal of materials science. Materials in medicine Vol. 25; no. 7; pp. 1701 - 1708
• Main Authors: Sankar, Renu, Dhivya, Ravishankar, Shivashangari, Kanchi Subramanian, Ravikumar, Vilwanathan
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.07.2014; Springer; Springer Nature B.V
• Subjects: Animals; Biological and medical sciences; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Ceramics; Chemistry and Materials Science; Composites; Diffraction; Electrophoresis, Polyacrylamide Gel; Glass; Hydrogen-Ion Concentration; Light; Light scattering; Male; Materials Science; Medical sciences; Metal Nanoparticles - chemistry; Microscopy, Electron, Scanning; Nanoparticles; Natural Materials; Origanum; Origanum vulgare; Particle Size; Plant Leaves - metabolism; Polymer Sciences; Rats; Rats, Wistar; Regenerative Medicine/Tissue Engineering; Rodents; Scattering, Radiation; Spectrophotometry, Ultraviolet; Spectroscopy, Fourier Transform Infrared; Surfaces and Interfaces; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Temperature; Thin Films; Time Factors; Tissue Engineering - methods; Titanium - chemistry; Titanium dioxide; Wound Healing; X-Ray Diffraction; Institutional Animal Ethic Committee; Titanium Dioxide Nanoparticles; Wound Closure; Metal Oxide Nanoparticles; TiO2; Titanium IV Oxides; Vertebrata; Mammalia; Rat; Nanoparticle; Animal; Rodentia; Cicatrization; Wound; Biomedical engineering
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-014-5193-5

The titanium dioxide nanoparticles (TiO2·NPs) were synthesized utilizing Origanum vulgare under room temperature. The green synthesized TiO2 NPs excitation was confirmed using UV–Vis spectrophotometer at 320 nm. Scanning electron microscopy analysis showed TiO2·NPs are spherical in shape and connected with one another. Dynamic light scattering analysis results specified high stability in nanoparticles, with an average particle size of 341 nm. Fourier transform infrared spectroscopy peaks revealed the presence of bioactive functional groups in Origanum vulgare aqueous leaf extract much needed for the TiO2·NPs formation. X-ray diffraction spectra showed the TiO2·NPs are amorphous in nature. Furthermore, the green synthesized TiO2·NPs wound healing activity was examined in the excision wound model by measuring wound closure, histopathology and protein profiling, revealed significant wound healing activity in Albino rats. In conclusion, our results bared TiO2·NPs have delivered a novel therapeutic route for wound treatment in clinical practice.