Inactivation of aflatoxin B1 by using the synergistic effect of hydrogen peroxide and gamma radiation

• Published in: Applied and Environmental Microbiology Vol. 55; no. 2; pp. 465 - 467
• Main Authors: Patel, U.D, Govindarajan, P, Dave, P
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.02.1989
• Subjects: 560130 - Radiation Effects on Microorganisms; 560300 - Chemicals Metabolism & Toxicology; AFLATOXIN; Aflatoxin B1; AFLATOXINAS; AFLATOXINE; Aflatoxins - antagonists & inhibitors; Aflatoxins - pharmacology; Aflatoxins - radiation effects; ALMACENAMIENTO; Animals; ASPERGILLUS; BACTERIA; Biological and medical sciences; CHEMICAL RADIATION EFFECTS; CHEMISTRY; CHROMATOGRAPHY; Dose-Response Relationship, Radiation; DOSE-RESPONSE RELATIONSHIPS; ELECTROMAGNETIC RADIATION; EUMYCOTA; Food industries; Food toxicology; Fundamental and applied biological sciences. Psychology; FUNGI; GAMMA RADIATION; Gamma Rays; HYDROGEN COMPOUNDS; HYDROGEN PEROXIDE; Hydrogen Peroxide - pharmacology; IONIZING RADIATIONS; LIQUID COLUMN CHROMATOGRAPHY; Microbiology; MICROORGANISMS; Microsomes, Liver - metabolism; MUTAGEN SCREENING; Mutagenicity Tests; Mycological methods and techniques used in mycology; Mycology; OXYGEN COMPOUNDS; PEROXIDES; PEROXIDO DE HIDROGENO; PEROXYDE D'HYDROGENE; PLANTS; RADIACION GAMMA; RADIATION CHEMISTRY; RADIATION EFFECTS; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT; RADIATIONS; RAYONNEMENT GAMMA; SALMONELLA; SALMONELLA TYPHIMURIUM; Salmonella typhimurium - drug effects; SCREENING; SEPARATION PROCESSES; SINERGISMO; STOCKAGE; SYNERGISM; SYNERGISME; TOXICITY; Chemosensitivity; Gamma irradiation; Radiosensitivity; Inhibition; Aflatoxin; Groundnut; HPLC chromatography; Detoxication; Inactivation; Hydrogen Peroxides; Synergism; Biological activity; Optimization; Fungi; Aspergillus; Thin layer chromatography; Aflatoxin B1; Fungi Imperfecti; Technique; Thallophyta
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.55.2.465-467.1989

Inactivation of aflatoxin B1 was studied by using gamma radiation and hydrogen peroxide. A 100-krad dose of gamma radiation was sufficient to inactivate 50 micrograms of aflatoxin B1 in the presence of 5% hydrogen peroxide, and 400 krad was required for total degradation of 100 micrograms of aflatoxin in the same system. Degradation of aflatoxin B1 was confirmed by high-pressure liquid chromatographic and thin-layer chromatographic analysis. Ames microsomal mutagenicity test showed loss of aflatoxin activity. This method of detoxification alos reduces the toxin levels effectively in artificially contaminated groundnuts