Identification of Rat Serum Alkaline Phosphatase Isoenzyme by means of Wheat Germ Agglutinin

Wheat germ agglutinin (WGA) precipitates bone type serum alkaline phosphatase (sALP) isoenzyme specifically. The precipitates are composed of the macromolecules of WGA and "bone type sALP" (WGA-ALP complex). In order to use bone type sALP as a marker in polyacrylamide gel electrophoresis (...

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Bibliographic Details
Published inExperimental Animals Vol. 46; no. 1; pp. 89 - 92
Main Authors WADA, Hiroshi, NIWA, Noboru, HAYAKAWA, Takashi, TSUGE, Haruhito
Format Journal Article
LanguageEnglish
Published Japan Japanese Association for Laboratory Animal Science 1997
Subjects
Alkaline Phosphatase - blood
Animals
Bone and Bones - enzymology
Chemical Precipitation
Electrophoresis, Polyacrylamide Gel
Isoenzymes - blood
Male
rat
Rats
serum alkaline phosphatase (sALP)
wheat germ agglutinin (WGA)
Wheat Germ Agglutinins
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Summary:Wheat germ agglutinin (WGA) precipitates bone type serum alkaline phosphatase (sALP) isoenzyme specifically. The precipitates are composed of the macromolecules of WGA and "bone type sALP" (WGA-ALP complex). In order to use bone type sALP as a marker in polyacrylamide gel electrophoresis (PAGE), a method to separate "bone type sALP" from the "WGA-ALP complex" was established by using N-acetyl-D-glucosamine (GlcNAc)-Sepharose 6B column chromatography. It was concluded that this method is useful for clinical examination in the rat.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1341-1357
1881-7122
DOI:10.1538/expanim.46.89