Detection of Escherichia coli Producing CTX-M-1-Group Extended-Spectrum β-Lactamases from Pigs in Aichi Prefecture, Japan, between 2015 and 2016

• Published in: Japanese Journal of Infectious Diseases Vol. 71; no. 1; pp. 33 - 38
• Main Authors: Norizuki, Chihiro, Kawamura, Kumiko, Wachino, Jun-ichi, Suzuki, Masahiro, Nagano, Noriyuki, Kondo, Takaaki, Arakawa, Yoshichika
• Format: Journal Article
• Language: English
• Published: Japan National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee 01.01.2018; Japan Science and Technology Agency
• Subjects: Amikacin; Animals; Anti-Bacterial Agents - therapeutic use; Bacteria; beta-Lactam Resistance - genetics; beta-Lactamases - genetics; beta-Lactamases - metabolism; Cefotaxime; Chloramphenicol; Ciprofloxacin; E coli; Epidemiology; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli Infections - epidemiology; Escherichia coli Infections - microbiology; Escherichia coli Infections - veterinary; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; extended-spectrum β-lactamase; Farms; Fosfomycin; Hogs; Imipenem; Japan - epidemiology; Microbial Sensitivity Tests - veterinary; molecular characterization; multilocus sequence typing; Multilocus Sequence Typing - veterinary; pig; Plasmids; Prevalence; Swine - microbiology; Swine Diseases - drug therapy; Swine Diseases - epidemiology; Swine Diseases - microbiology; Japan; extended-spectrum β-lactamase; multilocus sequence typing; prevalence; molecular characterization; pig
• ISSN: 1344-6304; 1884-2836
• DOI: 10.7883/yoken.JJID.2017.206

We investigated the prevalence and characteristics of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates from Japanese pigs. A total of 345 pig fecal specimens were collected from 30 farms in the Aichi prefecture of Japan between June 2015 and April 2016, and 22 unique ESBL-producing E. coli were isolated from 16 samples spanning 8 farms. The ESBL types included CTX-M-15 (54.5%), CTX-M-55 (27.2%), CTX-M-3 (0.9%), and CTX-M-14 (0.9%). The predominant plasmid replicon type was IncN, and the isolates carried blaCTX-M-55. Nine sequence type (ST)s, including ST117, ST1706, ST38, and ST10, were detected in the ESBL-producers, but no B2-O25-ST131 was found. ESBL producers were highly resistant to cefotaxime, ceftiofur, and tetracycline, but were susceptible to imipenem, amikacin, and fosfomycin (FOM), although 2 ST354 isolates showed resistance to ciprofloxacin. All 11 chloramphenicol-resistant isolates, including ST117 (n = 6) and ST38 (n = 3) isolates, harbored floR, and the 2 FOM-resistant ST38 isolates harbored fosA3. Our results suggest that pigs do not act as direct reservoirs in the transmission of ESBL genes to E. coli in humans. However, ST117 E. coli carrying IncN-type plasmids mediating blaCTX-M-55 were isolated from several different farms, suggesting the potential for future spread in Japan. Therefore, plasmid sequence analyses and continuous surveillance are necessary from an epidemiological point of view and are required to better protect against ESBL-producer transmission.