Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation

• Published in: The plant pathology journal Vol. 30; no. 1; pp. 96 - 101
• Main Authors: Koh, Hyun Seok, Kim, Gyoung Hee, Lee, Young Sun, Koh, Young Jin, Jung, Jae Sung
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국식물병리학회 01.03.2014; Korean Society of Plant Pathology; Hanrimwon Publishing Company
• Subjects: bacterial canker; kiwifruit; multiplex PCR; PCR detection; Pseudomonas syringae pv. actinidiae; 농학; multiplex PCR; PCR detection; bacterial canker; Pseudomonas syringae pv. actinidiae; kiwifruit
• ISSN: 1598-2254; 2093-9280
• DOI: 10.5423/PPJ.NT.09.2013.0095

The molecular features of Pseudomonas syringae pv. actinidiae strains isolated in Korea were compared with strains isolated in Japan and Italy. Sequencing of eight P. syringae pv. actinidiae and three P. syringae pv. theae strains revealed a total of 44 single nucleotide polymorphisms across 4,818 bp of the concatenated alignment of nine genes. A multiplex PCR assay was developed for the detection of P. syringae pv. actinidiae and for the specific detection of recent haplotype strains other than strains isolated since the 1980s in Korea. The primer pair, designated as TacF and TacR, specifically amplified a 545-bp fragment with the genomic DNA of new haplotype of P. syringae pv. actinidiae strains. A multiplex PCR conducted with the TacF/TacR primer pair and the universal primer pair for all P. syringae pv. actinidiae strains can be simultaneously applied for the detection of P. syringae pv. actinidiae and for the differentiation of new haplotype strains.