The clathrin adaptor Gga2p is a phosphatidylinositol 4-phosphate effector at the Golgi exit

Phosphatidylinositol 4-phosphate (PI(4)P) is a key regulator of membrane transport required for the formation of transport carriers from the trans-Golgi network (TGN). The molecular mechanisms of PI(4)P signaling in this process are still poorly understood. In a search for PI(4)P effector molecules,...

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Published inMolecular biology of the cell Vol. 19; no. 5; pp. 1991 - 2002
Main Authors Demmel, Lars, Gravert, Maike, Ercan, Ebru, Habermann, Bianca, Müller-Reichert, Thomas, Kukhtina, Viktoria, Haucke, Volker, Baust, Thorsten, Sohrmann, Marc, Kalaidzidis, Yannis, Klose, Christian, Beck, Mike, Peter, Matthias, Walch-Solimena, Christiane
Format Journal Article
LanguageEnglish
Published United States The American Society for Cell Biology 01.05.2008
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Summary:Phosphatidylinositol 4-phosphate (PI(4)P) is a key regulator of membrane transport required for the formation of transport carriers from the trans-Golgi network (TGN). The molecular mechanisms of PI(4)P signaling in this process are still poorly understood. In a search for PI(4)P effector molecules, we performed a screen for synthetic lethals in a background of reduced PI(4)P and found the gene GGA2. Our analysis uncovered a PI(4)P-dependent recruitment of the clathrin adaptor Gga2p to the TGN during Golgi-to-endosome trafficking. Gga2p recruitment to liposomes is stimulated both by PI(4)P and the small GTPase Arf1p in its active conformation, implicating these two molecules in the recruitment of Gga2p to the TGN, which ultimately controls the formation of clathrin-coated vesicles. PI(4)P binding occurs through a phosphoinositide-binding signature within the N-terminal VHS domain of Gga2p resembling a motif found in other clathrin interacting proteins. These data provide an explanation for the TGN-specific membrane recruitment of Gga2p.
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These authors contributed equally to this work.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.E06-10-0937