Glycan analysis of therapeutic glycoproteins

Therapeutic monoclonal antibodies (mAbs) are glycoproteins produced by living cell systems. The glycan moieties attached to the proteins can directly affect protein stability, bioactivity, and immunogenicity. Therefore, glycan variants of a glycoprotein product must be adequately analyzed and contro...

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Bibliographic Details
Published inmAbs Vol. 8; no. 2; pp. 205 - 215
Main Authors Zhang, Lei, Luo, Shen, Zhang, Baolin
Format Journal Article
LanguageEnglish
Published United States Taylor & Francis 17.02.2016
Subjects
Analytics
Animals
Antibodies, Monoclonal - analysis
Antibodies, Monoclonal - therapeutic use
biopharmaceuticals
glycan profiling
glycosylation
Humans
lectin microarray
Lectins - chemistry
monoclonal antibodies
Polysaccharides - analysis
Protein Array Analysis - methods
Review
therapeutic proteins
glycan profiling
lectin microarray
biopharmaceuticals
glycosylation
Analytics
therapeutic proteins
monoclonal antibodies
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Summary:Therapeutic monoclonal antibodies (mAbs) are glycoproteins produced by living cell systems. The glycan moieties attached to the proteins can directly affect protein stability, bioactivity, and immunogenicity. Therefore, glycan variants of a glycoprotein product must be adequately analyzed and controlled to ensure product quality. However, the inherent complexity of protein glycosylation poses a daunting analytical challenge. This review provides an update of recent advances in glycan analysis, including the potential utility of lectin-based microarray for high throughput glycan profiling. Emphasis is placed on comparison of the major types of analytics for use in determining unique glycan features such as glycosylation site, glycan structure, and content.
Bibliography:ObjectType-Article-2
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ObjectType-Review-1
ISSN:1942-0862
1942-0870
DOI:10.1080/19420862.2015.1117719