A comparison of native and non-urate Total Antioxidant Capacity of fasting plasma and saliva among middle-aged and older subjects

• Published in: Redox report : communications in free radical research Vol. 23; no. 1; pp. 57 - 62
• Main Authors: Gawron-Skarbek, Anna, Prymont-Przymińska, Anna, Sobczak, Agnieszka, Guligowska, Agnieszka, Kostka, Tomasz, Nowak, Dariusz, Szatko, Franciszek
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.01.2018; Taylor & Francis Group
• Subjects: Aged; Angiotensin-Converting Enzyme Inhibitors - therapeutic use; Antioxidants - analysis; Aspirin - therapeutic use; DPPH; Fasting - blood; Fasting - physiology; Female; FRAP; FRAS; Humans; Hypoglycemic Agents - therapeutic use; Male; Middle Aged; non-urate total antioxidant capacity; plasma; saliva; Saliva - chemistry; Saliva - metabolism; Total antioxidant capacity; uric acid; Uric Acid - analysis; Uric Acid - blood; saliva; Total antioxidant capacity; DPPH; non-urate total antioxidant capacity; FRAP; plasma; uric acid; FRAS
• ISSN: 1351-0002; 1743-2928
• DOI: 10.1080/13510002.2017.1392714

Objectives: As plasma and salivary total antioxidant capacity (TAC) is mainly contributed by uric acid (UA), the present study measures non-urate TAC (Nu-TAC). The aim of the study was to correlate plasma native TAC, Nu-TAC and UA with their salivary analogues, and compare the UA contribution in both body fluids using two different methods. Methods: The study involved 55 middle-aged and older subjects (66.7 ± 4.5 years). TAC was determined simultaneously with two methods (ferric reducing ability of plasma - FRAP, 2.2-diphenyl-1-picryl-hydrazyl - DPPH and countertypes for saliva - FRAS and DPPHS test), with and without UA (native TAC and Nu-TAC, respectively). Plasma UA and salivary UA (SUA) were assessed. Results: Subjects with increased FRAP, DPPH and UA had higher FRAS, DPPHS and SUA, respectively (P < 0.05). Plasma Nu-TAC indices did not correlate with salivary Nu-TAC. The contribution of UA to the plasma and salivary DPPH tests was similar: 75.7 ± 10.3% and 75.2 ± 14.0%, respectively. However, the contribution of UA to FRAS was higher than that for FRAP (71.6 ± 13.9% vs. 64.0 ± 8.1%; P < 0.001). Discussion: Our findings suggest that saliva is a good predictor for native plasma TAC but not for Nu-TAC. UA level is comparably dominant in saliva and in plasma according to DPPH, but lower in plasma according to FRAP.