Enzyme‐specific profiles for genome annotation: PRIAM
The advent of fully sequenced genomes opens the ground for the reconstruction of metabolic pathways on the basis of the identification of enzyme‐coding genes. Here we describe PRIAM, a method for automated enzyme detection in a fully sequenced genome, based on the classification of enzymes in the EN...
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Published in | Nucleic acids research Vol. 31; no. 22; pp. 6633 - 6639 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
Oxford University Press
15.11.2003
Oxford Publishing Limited (England) |
Subjects | |
Online Access | Get full text |
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Summary: | The advent of fully sequenced genomes opens the ground for the reconstruction of metabolic pathways on the basis of the identification of enzyme‐coding genes. Here we describe PRIAM, a method for automated enzyme detection in a fully sequenced genome, based on the classification of enzymes in the ENZYME database. PRIAM relies on sets of position‐specific scoring matrices (‘profiles’) automatically tailored for each ENZYME entry. Automatically generated logical rules define which of these profiles is required in order to infer the presence of the corresponding enzyme in an organism. As an example, PRIAM was applied to identify potential metabolic pathways from the complete genome of the nitrogen‐fixing bacterium Sinorhizobium meliloti. The results of this automated method were compared with the original genome annotation and visualised on KEGG graphs in order to facilitate the interpretation of metabolic pathways and to highlight potentially missing enzymes. |
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Bibliography: | local:gkg847 ark:/67375/HXZ-K15SHWG4-3 Received June 24, 2003; Revised August 26 2003;. Accepted September 23, 2003 istex:ED9EDA8E37658AEB0A2C862B184E68FF8E5F0009 To whom correspondence should be addressed Tel: +33 561285329; Fax: +33 561285061; Email: dkahn@toulouse.inra.fr ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0305-1048 1362-4962 1362-4962 |
DOI: | 10.1093/nar/gkg847 |