Reduced sensory stimulation alters the molecular make-up of glutamatergic hair cell synapses in the developing cochlea

• Published in: Neuroscience Vol. 325; pp. 50 - 62
• Main Authors: Barclay, M, Constable, R, James, N.R, Thorne, P.R, Montgomery, J.M
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 14.06.2016
• Subjects: Acoustic Stimulation; Animals; Auditory Pathways - growth & development; Auditory Pathways - metabolism; cochlea; Cochlea - growth & development; conductive deafness; development; DNA-Binding Proteins - metabolism; Evoked Potentials, Auditory, Brain Stem; glutamate; Hair Cells, Auditory, Inner - metabolism; Hair Cells, Auditory, Inner - pathology; Mice; Mice, Inbred C57BL; Nerve Tissue Proteins - metabolism; Neurology; Phosphoproteins - metabolism; Presynaptic Terminals - metabolism; Receptors, AMPA - metabolism; Sensory Deprivation; Spiral Ganglion - growth & development; Spiral Ganglion - metabolism; spiral ganglion neurons; synapses; cochlea; AMPA; α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor; excitatory postsynaptic potential; IHC; AMPA receptor subunit; normal goat serum; IgG (H + L); phosphate-buffered saline; GluA; ABR; auditory brainstem response; BSA; spiral ganglion neuron; glutamate; organ of Corti; PBS; development; EPSP; spiral ganglion neurons; inner hair cell; conductive deafness; synapses; oC; NGS; SGN; immunoglobulin (Heavy and Light chain); bovine serum albumin; IgG (H+L)
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2016.03.043

Highlights • Normal acoustic stimulation in the 2 weeks after the onset of hearing is not required for maintenance of afferent innervation of the inner hair cells in the cochlea. • Glutamatergic synapses in the cochleae are subject to activity-dependant plasticity after the onset of hearing. • The expression of RIBEYE in the presynaptic membrane and GluA2/3, GluA4 and Shank-1 in the type I SGN terminals are all affected by reduced acoustic stimulation.