Immune co-culture cell microarray - a feasible tool for high-throughput functional investigation of lymphocyte-cancer interactions

Omics analyses often result in dozens to hundreds of potential targets, requiring validation for their biological relevance. Current high-throughput functional investigation methods are frequently labor-intensive, expensive, and display low reproducibility. The Immune Co-Culture Cell Microarray (ICC...

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Published inOncoimmunology Vol. 9; no. 1; p. 1741267
Main Authors Baruch, Erez Nissim, Ortenberg, Rona, Avivi, Camila, Anafi, Liat, Dick-Necula, Daniela, Stossel, Chani, Moshkovits, Yonatan, Itzhaki, Orit, Besser, Michal Judith, Schachter, Jacob, Barshack, Iris, Markel, Gal
Format Journal Article
LanguageEnglish
Published United States Taylor & Francis 01.01.2020
Taylor & Francis Group
Subjects
Coculture Techniques
functional protein expression
Humans
immunological cytotoxicity test
Immunotherapy
Lymphocytes
Lymphocytes, Tumor-Infiltrating
melanoma
Neoplasms
omics validation
Original Research
Reproducibility of Results
functional protein expression
melanoma
Immunotherapy
omics validation
immunological cytotoxicity test
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Summary:Omics analyses often result in dozens to hundreds of potential targets, requiring validation for their biological relevance. Current high-throughput functional investigation methods are frequently labor-intensive, expensive, and display low reproducibility. The Immune Co-Culture Cell Microarray (ICCM) is a formalin-fixed paraffin-embedded cell block microarray based on co-cultures of patient-derived tumor-infiltrating lymphocytes and their autologous melanoma cells. Each ICCM slide represents the same experiment and can be stained using standard immunohistochemistry and immunofluorescence techniques. Functional dynamics assessment of both proteins and microRNAs using ICCM stained slides demonstrated similar findings to flow cytometry assays and to previously published patient-derived biopsy reports.
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These authors contributed equally to this work
ISSN:2162-4011
2162-402X
2162-402X
DOI:10.1080/2162402X.2020.1741267