Nitric oxide plays a key role in the suppressive activity of tolerogenic dendritic cells

• Published in: Cellular & molecular immunology Vol. 12; no. 3; pp. 384 - 386
• Main Authors: Verinaud, Liana, Issayama, Luidy Kazuo, Zanucoli, Fábio, de Carvalho, Ana Carolina, da Costa, Thiago Alves, Di Gangi, Rosária, Bonfanti, Amanda Pires, Ferreira, Isadora Tassinari, de Oliveira, Alexandre Leite Rodrigues, Machado, Dagmar Ruth Stach, Thomé, Rodolfo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.05.2015; Nature Publishing Group
• Subjects: Animals; Antibodies; Antimalarials - pharmacology; Biomedical and Life Sciences; Biomedicine; Cells, Cultured; Chloroquine - pharmacology; Dendritic cells; Dendritic Cells - immunology; Dendritic Cells - transplantation; Encephalomyelitis, Autoimmune, Experimental - immunology; Encephalomyelitis, Autoimmune, Experimental - therapy; Humans; Immune Tolerance; Immunology; Immunosuppression; Immunotherapy, Adoptive - methods; iNOS; Lymphocyte Activation; Medical Microbiology; Mice; Mice, Knockout; Microbiology; Multiple Sclerosis - immunology; Multiple Sclerosis - therapy; Nitric oxide; Nitric Oxide - immunology; Short Communication; T-Lymphocytes, Regulatory - immunology; Vaccine; 一氧化氮; 多发性硬化; 抑制活性; 树突细胞; 耐受性; 自身免疫性疾病; 调节性T细胞
• ISSN: 1672-7681; 2042-0226
• DOI: 10.1038/cmi.2014.94

Tolerogenic dendritic cells （DCs） are widely studied for their possible use in the treatment of inflammatory disorders, such as autoimmune diseases. One of the obstacles for the use of this cell-based therapy is the characterization of drugs that are able to modulate DCs. We have previously shown that chloroquine （CQ）, an antimalarial agent, has the ability to modulate DCs towards a tolerogenic phenotype.1 These tolerogenic DCs are able to suppress the development of experimental autoimmune encephalomyelitis （EAE）, a T cell-driven mouse model of human multiple sclerosis. In addition, several studies have proposed that nitric oxide （NO） plays a major role in the dif- ferentiation of regulatory T cells （Tregs） and the suppression of Thl/Th17 cells.2＂3 However, little is known about the role of DC-derived NO in the modulation of inflammatory auto- immune responses. Thus, we aimed to evaluate whether NO plays a role in the tolerogenic activity of CQ-treated DCs （CQ- DCs）. We found that CQ induces DC production of NO and expression of indoleamine 2,3-dioxygenase （IDO）, as well as inducible nitric oxide syrtthase （iNOS）.