Development of Specific Markers for Identification of Biovars 1 and 2 Strains of Pseudomonas syringae pv. Actinidiae

• Published in: The plant pathology journal Vol. 32; no. 2; pp. 162 - 167
• Main Authors: Lee, Young Sun, Kim, Gyoung Hee, Koh, Young Jin, Zhuang, Qiguo, Jung, Jae Sung
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국식물병리학회 01.04.2016; Korean Society of Plant Pathology; Hanrimwon Publishing Company
• Subjects: bacterial canker; biovar; kiwifruit; molecular marker; Pseudomonas syringae pv. actinidiae; 농학; Pseudomonas syringae pv. Actinidiae; biovar; bacterial canker; molecular marker; kiwifruit; Pseudomonas syringae pv. actinidiae
• ISSN: 1598-2254; 2093-9280
• DOI: 10.5423/PPJ.NT.10.2015.0224

Pseudomonas syringae pv. actinidiae, the causal agent of canker in kiwifruit, can be divided into three biovars (biovars 1, 2, and 3). Strains belonging to biovar 1 produce phaseolotoxin and were isolated in Japan and Italy before 2008. Strains of biovar 2 produce coronatine instead of phaseolotoxin and have been isolated only in Korea. Strains belonging to biovar 3 produce neither phaseolotoxin nor coronatine and are responsible for the global outbreak of bacterial canker of kiwifruit in recent years. The biovar 3-specific primer set was developed in a previous work. In this study, two sets of PCR primers specific to strains of biovars 1 and 2, respectively, were developed based on random amplified polymorphic DNA analyses. Primers PsaJ-F and PsaJ-R produced a 481-bp region with genomic DNA of biovar 1 strains, whereas primers PsaK-F and PsaK-R amplified a 413-bp region present only in the genome of biovar 2 strains.