cpSecA, a thylakoid protein translocase subunit, is essential for photosynthetic development in Arabidopsis

• Published in: Journal of experimental botany Vol. 61; no. 6; pp. 1655 - 1669
• Main Authors: Liu, Dong, Gong, Qingqiu, Ma, Yuanyuan, Li, Pengli, Li, Jinping, Yang, Shuhua, Yuan, Lingling, Yu, Yunqing, Pan, Dadi, Xu, Fan, Wang, Ning Ning
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.06.2010
• Subjects: adenosinetriphosphatase; Albino; Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis - physiology; Arabidopsis - ultrastructure; Arabidopsis Proteins; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; AtcpSecA; Biological and medical sciences; Biosynthesis; carbon; Chlorophylls; chloroplast biogenesis; Chloroplast Proteins; Chloroplasts; Dehydrogenases; electron microscopy; Electrophoresis, Polyacrylamide Gel; enzymology; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Regulation, Plant; Gene Expression Regulation, Plant - genetics; Gene Expression Regulation, Plant - physiology; Genes; genetics; Membrane Proteins; Membrane Proteins - genetics; Membrane Proteins - metabolism; metabolism; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; mutants; Oligonucleotide Array Sequence Analysis; oxidative stress; Oxidative Stress - genetics; Oxidative Stress - physiology; physiology; Plant cells; Plant Proteins; Plant Proteins - genetics; Plant Proteins - metabolism; Plants; Plants, Genetically Modified; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Plants, Genetically Modified - physiology; Plants, Genetically Modified - ultrastructure; Plastids; protein subunits; Protein Subunits - genetics; Protein Subunits - metabolism; protein transport; proteins; Research Papers; retrograde signal; Reverse Transcriptase Polymerase Chain Reaction; Seedlings; Thylakoids; Thylakoids - enzymology; Thylakoids - ultrastructure; transgenic plants; ultrastructure; Albinism; Arabidopsis; retrograde signal; Subunit; Thylakoid; Protein; Arabidopsis thaliana; AtcpSecA; Cruciferae; Dicotyledones; Angiospermae; Botany; Development; Albino; chloroplast biogenesis; Spermatophyta; Experimental plant; Photosynthesis; Chloroplast
• ISSN: 0022-0957; 1460-2431; 1460-2431
• DOI: 10.1093/jxb/erq033

The endosymbiont-derived Sec-dependent protein sorting pathway is essential for protein import into the thylakoid lumen and is important for the proper functioning of the chloroplast. Two loss-of-function mutants of cpSecA, the ATPase subunit of the chloroplast Sec translocation machinery, were analysed in Arabidopsis. The homozygous mutants were albino and seedling lethal under autotrophic conditions and remained dwarf and infertile with an exogenous carbon supply. They were subject to oxidative stress and accumulated superoxide under normal lighting conditions. Electron microscopy revealed that the chloroplast of the mutants had underdeveloped thylakoid structures. Histochemical GUS assay of the AtcpSecA::GUS transgenic plants confirmed that AtcpSecA was expressed in green organs in a light-inducible way. Real-time RT-PCR and microarray analysis revealed repressed transcription of nucleus- and chloroplast- encoded subunits of photosynthetic complexes, and induced transcription of chloroplast protein translocation machinery and mitochondrion-encoded respiratory complexes in the mutants. It is inferred that AtcpSecA plays an essential role in chloroplast biogenesis, the absence of which triggered a retrograde signal, eventually leading to a reprogramming of chloroplast and mitochondrial gene expression.