Cloning and characterization of a gene complementing the mutation of an ethanol-sensitive mutant of Sake yeast [Saccharomyces cerevisiae]

• Published in: Bioscience, biotechnology, and biochemistry Vol. 64; no. 2; pp. 229 - 236
• Main Authors: Inoue, T. (Nishinomiya Syuzo Co. Ltd., Hyogo (Japan)), Iefuji, H, Fujii, T, Soga, H, Satoh, K
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.02.2000; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; CLONACION; CLONAGE; CLONING; Cloning, Molecular; Drug Tolerance - genetics; ERG6; ERGOSTEROL; Ergosterol - analysis; ETANOL; ETHANOL; Ethanol - pharmacology; ethanol-sensitive mutant; FERMENTACION; FERMENTATION; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genetic Complementation Test; Genetics; Methyltransferases - genetics; Mission oriented research; MUTANT; MUTANTES; MUTANTS; Mutation; Oryza; SACCHAROMYCES CEREVISIAE; TRANSFERASAS; TRANSFERASE; TRANSFERASES; Wine - microbiology; Yeasts - chemistry; Yeasts - drug effects; Yeasts - genetics; Ergosterol; Ethanol; Enzyme; Transferases; Biosynthesis; Fungi; Sensitivity; Methyltransferases; 24-Sterol C-methyltransferase; Ascomycetes; Complementation; Sake yeast; Mutation; Molecular cloning; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.64.229

Ethanol-sensitive mutants (es1 to es10) were isolated from sake yeast, Saccharomyces cerevisiae SY-32. These mutants were unable to grow at 7% ethanol at which the wild type strain SY-32 does grow. The mutants had a variety of fermentation rates and viabilities in the presence of ethanol. The gene ERG6, complementing the ethanol-sensitive mutation of es5, was cloned from an SY-32 gene library. ERG6 encodes S-adenosylmethionine: delta 24-sterol-C-methyltransferase (EC 2.1.1.41) in the ergosterol synthetic pathway. Mutant es5 had a reduced ability to synthesize ergosterol. An erg6 disruptant was also ethanol-sensitive. These results suggested that ERG6 plays an important role in the ethanol tolerance of S. cerevisiae