Long noncoding RNA MEG3 activation of p53 mediates ischemic neuronal death in stroke

• Published in: Neuroscience Vol. 337; pp. 191 - 199
• Main Authors: Yan, Honglin, Yuan, Jingping, Gao, Likun, Rao, Jie, Hu, Jichang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 19.11.2016
• Subjects: Animals; Cell Death; Cell Line, Tumor; Cell Proliferation - genetics; Gene Expression Regulation, Neoplastic; ischemia; Male; maternally expressed gene 3; Mice, Inbred C57BL; Neurology; neuronal death; p53; Promoter Regions, Genetic - genetics; RNA, Long Noncoding - genetics; Stroke - genetics; Tat-p53-DBD271–282; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism; MEG3; microRNAs; magnetic resonance imaging; TTC; Tat-p53-DBD 271–282; lncRNA; FJ; miRNAs; MRI; piwi-associated RNAs; CNS; piRNAs; Fluoro-Jade C; siRNAs; p53; preinitiation complex; propidium iodide; RNS; reactive oxygen species; internal carotid artery; PDL; reactive nitrogen species; MCAO; oxygen glucose deprivation; small interfering RNAs; ECA; ICA; qRT-PCR; MCA; PIC; quantitative real-time PCR; OGD; middle cerebral artery; central nervous system; ischemia; maternally expressed gene 3; poly- d-lysine; neuronal death; ROS; PI; long non-coding RNA; 2,3,5-triphenyltetrazolium chloride; external carotid artery; middle cerebral artery occlusion; Tat-p53-DBD271–282; Tat-p53-DBD(271–282)
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2016.09.017

Highlights • We first confirmed the expression and function of long noncoding RNA maternally expressed gene 3 (MEG3) in ischemia. • MEG3 binds with p53-DBD271–282 which stimulates p53-mediated transactivation and mediates ischemic neuronal death. • Intervention of MEG3–p53 interaction by administration of Tat-p53-DBD271–282 protects against ischemic insults.