The prebiotic effects of omega-3 fatty acid supplementation: A six-week randomised intervention trial

• Published in: Gut microbes Vol. 13; no. 1; pp. 1 - 11
• Main Authors: Vijay, Amrita, Astbury, Stuart, Le Roy, Caroline, Spector, Tim D, Valdes, Ana M
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.01.2021; Taylor & Francis Group
• Subjects: Aged; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bacteria - metabolism; Body Mass Index; Dietary intervention; Dietary Supplements; Fatty Acids - blood; Fatty Acids, Omega-3 - administration & dosage; Fatty Acids, Omega-3 - pharmacology; Female; Fibre; Gastrointestinal Microbiome - drug effects; Gut microbiota; Humans; Inulin - administration & dosage; Inulin - pharmacology; Lipids - blood; Male; Middle Aged; Omega 3; Prebiotic; Prebiotics - administration & dosage; Research Paper; Gut microbiota; Omega 3; Fibre; Dietary intervention; Prebiotic
• ISSN: 1949-0976; 1949-0984
• DOI: 10.1080/19490976.2020.1863133

Prebiotics are compounds in food that benefit health via affecting the gut microbiome. Omega-3 fatty acids have been associated with differences in gut microbiome composition and are widely accepted to have health benefits, although recent large trials have been inconclusive. We carried out a 6-week dietary intervention comparing the effects of daily supplementation with 500 mg of omega-3 versus 20 g of a well-characterized prebiotic, inulin. Inulin supplementation resulted in large increases in Bifidobacterium and Lachnospiraceae. In contrast, omega-3 supplementation resulted in significant increases in Coprococcus spp. and Bacteroides spp, and significant decreases in the fatty-liver associated Collinsella spp. On the other hand, similar to the results with inulin supplementation which resulted in significant increases in butyrate, iso-valerate, and iso-butyrate (p < .004), omega-3 supplementation resulted in significant increases in iso-butyrate and isovalerate (p < .002) and nearly significant increases in butyrate (p < .053). Coprococcus, which was significantly increased post-supplementation with omega-3, was found to be positively associated with iso-butyric acid (Beta (SE) = 0.69 (0.02), P = 1.4 x 10 −3 ) and negatively associated with triglyceride-rich lipoproteins such as VLDL (Beta (SE) = −0.381 (0.01), P = .001) and VLDL-TG (Beta (SE) = −0.372 (0.04), P = .001) after adjusting for confounders. Dietary omega-3 alters gut microbiome composition and some of its cardiovascular effects appear to be potentially mediated by its effect on gut microbial fermentation products indicating that it may be a prebiotic nutrient.