FOXD3 inhibits cell proliferation, migration, and invasion in nasopharyngeal carcinoma through regulation of the PI3K–Akt pathway

• Published in: Biochemistry and cell biology Vol. 98; no. 6; pp. 653 - 660
• Main Authors: Xie, Xiaoxing, Xiong, Gaoyun, Chen, Wenjun, Fu, Hongdan, Li, Mingqian, Cui, Xiaoying
• Format: Journal Article
• Language: English
• Published: 1840 Woodward Drive, Suite 1, Ottawa, ON K2C 0P7 NRC Research Press 01.11.2020
• Subjects: Cancer invasiveness; carcinome nasopharyngien (CNP); Cell migration; Cell proliferation; Cellular signal transduction; Control; FOXD3; Nasopharyngeal cancer; nasopharyngeal carcinoma (NPC); Physiological aspects; PI3K–Akt pathway; voie PI3K–Akt; China; voie PI3K–Akt; FOXD3; PI3K–Akt pathway; nasopharyngeal carcinoma (NPC); carcinome nasopharyngien (CNP)
• ISSN: 0829-8211; 1208-6002
• DOI: 10.1139/bcb-2020-0011

FOXD3 has been found previously to positively regulate miR-26b, a tumor inhibitor of nasopharyngeal carcinoma (NPC). However, FOXD3’s precise function and associated mechanism of action in NPC have not yet been investigated. In this study, the expression of FOXD3 mRNA and protein was evaluated using RT-qPCR, western blotting, and immunohistochemistry. Protein levels involved in the phosphoinositide 3-kinase – protein kinase B (PI3K–Akt) pathway were assessed by western blot, and cell proliferation was determined by MTT and colony forming assays. Additionally, cell apoptosis was assessed by flow cytometric assay. Finally, the migration and invasion capabilities of the NPC cells were determined using wound healing and Transwell assays. We found that FOXD3 levels were relatively low in NPC tissue and cells, while an increase caused the inhibition of the PI3K–Akt pathway. Functional experiments found that overexpression of FOXD3 suppressed cell proliferation, migration, and invasion and enhanced cell apoptosis in NPC C6661 cells. IGF-1, an activator of the PI3K–Akt pathway, reversed the inhibitory effect of FOXD3. Furthermore, we found upregulation of the PI3K–Akt pathway and upregulation of the inhibitory effects of FOXD3 on C6661 cellular activities. In conclusion, FOXD3 negatively affected the PI3K–Akt pathway to restrain the processes involved in C6661 cell pathology. These findings further exposed the function and downstream axis of FOXD3 in NPC and displayed a promising new target for NPC therapy.