Chemical ligation of oligodeoxynucleotides by X-irradiation and its application to regulation of G-quadruplex formation

We demonstrated radiolytic ligation of oligodeoxynucleotides (ODNs) possessing disulfide bond and its application to regulation of DNA quadruplex formation. G-rich hexamer ODNs had poor ability to form quadruplex, while X-irradiation of the ODNs induced interstrand exchange reaction at disulfide bon...

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Published inBioorganic & medicinal chemistry letters Vol. 23; no. 7; pp. 2098 - 2100
Main Authors Tanabe, Kazuhito, Mizoe, Taiga, Sugiura, Masaaki, Okada, Kana, Nishimoto, Sei-ichi
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.04.2013
Subjects
absorption
Chemical ligation
Chemiluminescence
disulfide bonds
DNA
DNA - chemistry
DNA quadruplex
G-Quadruplexes
Nucleic Acid Conformation
oligodeoxyribonucleotides
Oligodeoxyribonucleotides - chemistry
oxidation
potassium
X-Irradiation
X-Rays
Chemiluminescence
DNA quadruplex
X-Irradiation
Chemical ligation
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Summary:We demonstrated radiolytic ligation of oligodeoxynucleotides (ODNs) possessing disulfide bond and its application to regulation of DNA quadruplex formation. G-rich hexamer ODNs had poor ability to form quadruplex, while X-irradiation of the ODNs induced interstrand exchange reaction at disulfide bond to form ligated 12 mer ODNs, leading to the ready formation of quadruplex due to the entropic effect. Since complexation of the ligated ODNs with hemin in the presence of K+ showed strong soret band absorption and also catalyzed the H2O2-mediated oxidation of luminol, it appears that the quadruplex formed from ligated ODNs showed a function similar to native DNA quadruplex.
Bibliography:http://dx.doi.org/10.1016/j.bmcl.2013.01.123
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0960-894X
1464-3405
DOI:10.1016/j.bmcl.2013.01.123