Temporal variation in methanogen communities of four different full-scale anaerobic digesters treating food waste-recycling wastewater

• Published in: Bioresource technology Vol. 168; pp. 59 - 63
• Main Authors: Lee, Joonyeob, Hwang, Byungchul, Koo, Taewoan, Shin, Seung Gu, Kim, Woong, Hwang, Seokhwan
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Kidlington Elsevier Ltd 01.09.2014; Elsevier
• Subjects: 454 pyrosequencing; Anaerobic digestion; Anaerobiosis; Applied sciences; Archaea - metabolism; Bacteria - metabolism; Biological and medical sciences; Biological treatment of sewage sludges and wastes; Bioreactors - microbiology; Biotechnology; Environment and pollution; Exact sciences and technology; Food; Food industries; Food waste-recycling wastewater; Full-scale; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; Methane - metabolism; Methanogen community; Pollution; Recycling; Sequence Analysis, DNA; Temperature; Time Factors; Use and upgrading of agricultural and food by-products. Biotechnology; Waste Water - microbiology; Wastes; Wastewaters; Water Purification - instrumentation; Water treatment and pollution; Food waste-recycling wastewater; 454 pyrosequencing; Full-scale; Methanogen community; Anaerobic digestion; Sewage digester; Waste treatment; Chemiluminescence; Time variation; Waste water; Food waste; Methanogenic bacteria; Waste reuse; Sequencing; Community
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2014.03.161

•Methanoculleus was dominant in the plug flow thermophilic digester.•Methanoculleus and Methanothermobacter were dominant in the thermophilic CSTR.•Methanosaeta was dominant in the UASB mesophilic digester.•Methanoculeus and Methanosaeta were dominant in the mesophilic CSTR. Methanogen communities were investigated using 454 pyrosequencing in four different full-scale anaerobic digesters treating food waste-recycling wastewater. Seasonal samples were collected for 2years, and 24 samples were available for microbial analysis from a plug flow thermophilic (PT) digester, a continuously-stirred tank thermophilic (CT) digester, an upflow anerobic sludge blanket mesophilic (UM) digester, and a continuously-stirred tank mesophilic (CM) digester. Methanoculleus, Methanobacterium, Methanothermobacter, and Methanosaeta were revealed to be key methanogens in full-scale anaerobic digestion process treating food waste-recycling wastewater. In the PT digester, Methanoculleus was dominant (96.8%). In the CT digester, Methanoculleus was dominant (95.4%) during the first year of operation, but the dominant genus was shifted to Methanothermobacter (98.5%) due to pH increase. In the UM digester, Methanosaeta was dominant (87.2%). In the CM digester, Methanoculleus was constantly dominant (74.8%) except during CM5 when Methanosaeta was dominant (62.6%) due to the low residual acetate concentration (0.1g/L).