ITS-PCR-RFLP Method for Distinguishing Commercial Cultivars of Edible Mushroom, Flammulina velutipes

• Published in: Journal of food science Vol. 67; no. 7; pp. 2486 - 2490
• Main Authors: Palapala, V.A, Aimi, T, Inatomi, S, Morinaga, T
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.2002; Institute of Food Technologists; Wiley Subscription Services, Inc
• Subjects: Biological and medical sciences; cultivars; Deoxyribonucleic acid; DNA; edible mushroom; Enzymes; Flammulina velutipes; Food industries; Food science; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; General aspects; Genes; internal transcribed spacer; internal transcribed spacers; ITS; Methods of analysis, processing and quality control, regulation, standards; Mushrooms; PCR-RFLP; polymerase chain reaction; rDNA; restriction endonucleases; restriction fragment length polymorphism; ribosomal DNA; ribosomal RNA; rRNA 5.8S; Fungi; Polymerase chain reaction; Analysis method; Restriction fragment length polymorphism; Quality control; Ribosomal DNA; Basidiomycetes; Identification; Molecular biology; Edible fungi; Thallophyta; Cultivar
• ISSN: 0022-1147; 1750-3841
• DOI: 10.1111/j.1365-2621.2002.tb08763.x

Nucleotide sequence and polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLP) analysis of the ribosomal RNA gene (rDNA) regions containing the internal transcribed spacers (ITSs) and the 5.8S rRNA coding sequence was used to differentiate between 7 typical Flammulina strains. These nucleotide sequences revealed the presence of strain-specific deletions, insertions, and substitutions. Moreover, RFLP patterns produced using restriction endonucleases DraI, FokI, HaeII, MboII, and NlaIV, enabled identification of specific Flammulina strains. Thus, PCR-RFLP analysis of the ITS regions appears to be a useful tool for the identification of Flammulina strains.